The Yeast Two-hybrid System

The Yeast Two-hybrid System

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The yeast two-hybrid system is among the most powerful and generally useful methods for screening genes that are functional in various physiological processes and systems, and for determining gene interactions. Fields is one of the pioneers and leading practitioners of this approach, and the volume provides an accessible, critical summary of the methods and range of applications of gene screening possible with the yeast two-hybrid system.
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Product details

  • Paperback | 356 pages
  • 154.2 x 234.4 x 17.3mm | 498.96g
  • Oxford University Press Inc
  • New York, United States
  • English
  • halftones, line figures, tables
  • 0195109384
  • 9780195109382

Review quote

"Since the development of the yeast two-hybrid system by Fields and colleagues, it has developed into one of the most popular tools in biology. So much so that it has become difficult to follow the numerous modifications, improvements and multitudinous applications of the technique. The consolidation of all this information into a single volume, The Yeast Two-Hybrid System, will now make it much easier for interested researchers to come to grips with the technology." --Nature Structural Biology"The Yeast Two-Hybrid System is the first book of its kind with comprehensive coverage on both principles and practice of this powerful genetic system. For beginners, the book can serve as a useful introduction to the technique. For those who are already using one version or another of the system, the book will help to broaden their perspective. It can be used as either a lab manual or a general reference book. Almost everything one needs to know about the yeast two-hybrid system can be found in this book." --Nature Structural Biology"This book is intended for the wide range of biologists interested in using the 'Two-Hybrid System' to identify proteins that interact. . . . Part I contains 4 chapters that describe the most common variations of the two-hybrid system, complete with details about plasmid structures and yeast strain genotypes. There is sufficient information in these chapters for the average molecular biologist to implement the technique. . . . This book is a great resource for beginners as well as those who already use this technique, because each chapter contains its own methods section. This allows one to compare strategies that have evolved separately in different labs. Furthermore, the book was edited by Stanley Fields, the inventor of the Two-Hybrid System, and the chapters were written by the workers who helped refine the methodology. It is a useful guide written by successful practitioners."--The Quarterly Review of Biology "Since the development of the yeast two-hybrid system by Fields and colleagues, it has developed into one of the most popular tools in biology. So much so that it has become difficult to follow the numerous modifications, improvements and multitudinous applications of the technique. The consolidation of all this information into a single volume, The Yeast Two-Hybrid System, will now make it much easier for interested researchers to come to grips with the technology." --Nature Structural Biology "The Yeast Two-Hybrid System is the first book of its kind with comprehensive coverage on both principles and practice of this powerful genetic system. For beginners, the book can serve as a useful introduction to the technique. For those who are already using one version or another of the system, the book will help to broaden their perspective. It can be used as either a lab manual or a general reference book. Almost everything one needs to know about the yeast two-hybrid system can be found in this book." --Nature Structural Biology "This book is intended for the wide range of biologists interested in using the 'Two-Hybrid System' to identify proteins that interact. . . . Part I contains 4 chapters that describe the most common variations of the two-hybrid system, complete with details about plasmid structures and yeast strain genotypes. There is sufficient information in these chapters for the average molecular biologist to implement the technique. . . . This book is a great resource for beginners as well as those who already use this technique, because each chapter contains its own methods section. This allows one to compare strategies that have evolved separately in differentlabs. Furthermore, the book was edited by Stanley Fields, the inventor of the Two-Hybrid System, and the chapters were written by the workers who helped refine the methodology. It is a useful guide written by successful practitioners."--The Quarterly Review of Biology "Since the development of the yeast two-hybrid system by Fields and colleagues, it has developed into one of the most popular tools in biology. So much so that it has become difficult to follow the numerous modifications, improvements and multitudinous applications of the technique. The consolidation of all this information into a single volume, The Yeast Two-Hybrid System, will now make it much easier for interested researchers to come to grips with the technology." --Nature Structural Biology "The Yeast Two-Hybrid System is the first book of its kind with comprehensive coverage on both principles and practice of this powerful genetic system. For beginners, the book can serve as a useful introduction to the technique. For those who are already using one version or another of the system, the book will help to broaden their perspective. It can be used as either a lab manual or a general reference book. Almost everything one needs to know about the yeast two-hybrid system can be found in this book." --Nature Structural Biology "This book is intended for the wide range of biologists interested in using the 'Two-Hybrid System' to identify proteins that interact. . . . Part I contains 4 chapters that describe the most common variations of the two-hybrid system, complete with details about plasmid structures and yeast strain genotypes. There is sufficient information in these chapters for the average molecular biologist to implement the technique. . . . This book is a great resource for beginners as well as those who already use this technique, because each chapter contains its own methods section. This allows one to comparestrategies that have evolved separately in different labs. Furthermore, the book was edited by Stanley Fields, the inventor of the Two-Hybrid System, and the chapters were written by the workers who helped refine the methodology. It is a useful guide written by successful practitioners."--The Quarterly Review of Biology "Since the development of the yeast two-hybrid system by Fields and colleagues, it has developed into one of the most popular tools in biology. So much so that it has become difficult to follow the numerous modifications, improvements and multitudinous applications of the technique. Theconsolidation of all this information into a single volume, The Yeast Two-Hybrid System, will now make it much easier for interested researchers to come to grips with the technology." --Nature Structural Biology"The Yeast Two-Hybrid System is the first book of its kind with comprehensive coverage on both principles and practice of this powerful genetic system. For beginners, the book can serve as a useful introduction to the technique. For those who are already using one version or another of the system, the book will help to broaden their perspective. It can be used as either a lab manual or a general reference book. Almost everything one needs to know about the yeast two-hybrid system can be found in this book." --Nature Structural Biology"This book is intended for the wide range of biologists interested in using the 'Two-Hybrid System' to identify proteins that interact. . . . Part I contains 4 chapters that describe the most common variations of the two-hybrid system, complete with details about plasmid structures and yeast straingenotypes. There is sufficient information in these chapters for the average molecular biologist to implement the technique. . . . This book is a great resource for beginners as well as those who already use this technique, because each chapter contains its own methods section. This allows one tocompare strategies that have evolved separately indifferent labs. Furthermore, the book was edited by Stanley Fields, the inventor of the Two-Hybrid System, and the chapters were written by the workers who helped refine the methodology. It is a useful guide written by successfulpractitioners."--The Quarterly Review of Biology
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