Yeast Protocols

Yeast Protocols

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In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
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Product details

  • Hardback | 392 pages
  • 160 x 236.2 x 30.5mm | 793.8g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2006
  • 104 Illustrations, black and white; XII, 392 p. 104 illus.
  • 1588294374
  • 9781588294371

Back cover copy

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred during the last decade to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research. Yeast Protocols, Second Edition contains 27 optimized and readily reproducible techniques suitable for both yeast researchers and investigators who wish to use yeast to study their favorite genes from other organisms. The authors provide detailed methods for the isolation of subcellular components, including organelles and macromolecules; for the basic cellular and molecular analysis specific for yeast cells; and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Yeast Protocols, Second Edition offers researchers a collection of traditional and novel protocols essential for studying yeasts, as well as genes from other organisms.
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Review quote

From Reviews of the First Edition...

"...will benefit the apprentices...and the experienced scientist venturing into a new technique or changing yeast species...should join the other 'cookery' books on the laboratory shelf." -Journal of Emergency Medicine

"...a useful addition to life sciences libraries and to laboratories embarking on studies with yeast"
-Quarterly Review of Biology
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Table of contents

Basic Investigations in Saccharomyces cerevisiae
Brendan P. G. Curran and Virginia Bugeja

Isolation of Nucleic Acids
Michelle Hanna and Wei Xiao

Purification of Yeast Peroxisomes
Ben Distel and Astrid Kragt

Isolation of Yeast Plasma Membranes
Barry Panaretou and Peter Piper

Isolation of Yeast Mitochondria
Chris Meisinger, Nikolaus Pfanner, and Kaye N. Truscott

Extraction of Yeast Lipids
Roger Schneiter and Gunther Daum

Two-Dimensional Gel Electrophoresis of Total Yeast Proteins
Helian Boucherie and Christelle Monribot-Espagne

Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes
Laura Maringele and David Lydall. Analysis of Yeast Lipids, Roger Schneiter and Gunther Daum

Yeast Fluorescence Microscopy
Jiri Hasek

Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe
Michelle A. Alting-Mees, Eddy P. Risseeuw, Enwu Liu, Michel Desautels, William A. Crosby, and Sean M. Hemmingsen

Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
R. Daniel Gietz and Robin A. Woods

Mutagenesis
Leslie Barbour, Michelle Hanna, and Wei Xiao

Gene Disruption in the Budding Yeast Saccharomyces cerevisiae
Johannes H. Hegemann, Ulrich Guldener, and Gabriele J. Koehler

Inducible Degron and Its Application to Creating Conditional Mutants
R. Jurgen Dohmen

Synthetic Lethal Screen
Leslie Barbour and Wei Xiao

Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
Amy Hin Yan Tong and Charles Boone

Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
Alain T. Dandjinou, Michel Larrivee, Ralf E. Wellinger, and Raymund J. Wellinger

Chromatin Assembly in a Crude Fraction From Yeast Cells
Karen M.Robinson and Michael C. Schultz

Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast
Elena Ezhkova and William P. Tansey

Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning
Zhengjian Zhang and Joseph C. Reese

Study of Transcriptional Regulation Using a Reporter Gene Assay
Yu Fu and Wei Xiao

Assessing Telomeric Phenotypes
Catherine LeBel, Michel Larrivee, Amadou Bah, Nancy Laterreur, Nancy Levesque, and Raymund J. Wellinger

Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
Zhigang Wang

Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D Carrier
Marja Makarow, Anna-Liisa Hanninen, Taina Suntio, and Ricardo Nunes Bastos

Yeast Two-Hybrid System Screening
R. Daniel Gietz

Import of Precursor Proteins Into Isolated Yeast Mitochondria
Nils Wiedemann, Nikolaus Pfanner, and Peter Rehling

Index
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