In Vitro Transcription and Translation Protocols

In Vitro Transcription and Translation Protocols

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A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are more

Product details

  • Hardback | 276 pages
  • 154.9 x 231.1 x 27.9mm | 567g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2007
  • 23 Tables, black and white; 1 Illustrations, color; 50 Illustrations, black and white; XII, 276 p. 51 illus., 1 illus. in color.
  • 1588295583
  • 9781588295583

Table of contents

Section One: TECHNOLOGIES Chapter One - Energy Systems for ATP Regeneration in Cell-free Protein Synthesis Reactions Kara A. Calhoun, James R. Swartz Chapter 2 - Continuous-exchange Protein-synthesizing Systems Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin Chapter 3 - Cell-free production of integral membrane proteins on a preparative scale' Christian Klammt, Daniel Schwarz, Vilker Dotsch, Frank Bernhard Chapter 4- SIMPLEX: Single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano Chapter 5 - Methods for High-throughput Materialization of Genetic Information Based on Wheat Germ Cell-free Expression System Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo Chapter 6 - Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti Section Two: APPLICATIONS Chapter 7 -In vitro translation to study HIV protease activity Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama, Hirohito Tsubouchi Chapter 8 - The protein truncation test in mutation detection and molecular diagnosis Oliver Hauss, Oliver Muller Chapter 9 - Creation of Novel Enantioselective lipases by SIMPLEX Yuichi Koga, Tsuneo Yamane, Hideo Nakano Chapter 10 - In vitro transcription and translation coupled to 2D-electrophoresis for bacterial proteome analysis Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi Chapter 11 - In vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation and Immunoprecipitation Analysis Orit Gat, Haim Grosfeld,Avigdor Shafferman Chapter 12 - Functional expression of type 1 rat GABA transporter in microinjected Xenopus laevis oocytes Stefano Giovannardi, Simona Magagnin, Andrea Soragna, Laura Faravelli Chapter 13 - Production of Protein for NMR Study Using the Wheat Germ Cell-Free System Toshiyuki Kohno, Yaeta Endoshow more