Virology: A Laboratory Manual (Instructor's Manual)

Virology: A Laboratory Manual (Instructor's Manual)

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Description

This instructor's manual accompanies the main text, which is designed for a one-semester virology laboratory course.
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Product details

  • Paperback | 17 pages
  • 152 x 229 x 6.35mm | 60g
  • Academic Press Inc
  • San Diego, United States
  • English
  • Teacher’s edition
  • Teachers Guide
  • 0121447316
  • 9780121447311

Table of contents

Introduction to the Virology Laboratory.
Preparation of Cell Culture Medium.
Cell Growth and Cell Passage.
Counting Cells with a Hemacytometer.
Primary Cell Cultures.
Cryopreservation of Cell Lines.
Virus Growth in Cell Culture.
Virus Growth in Embroynated Eggs.
TCID50.
LD50.
EID50 Assay.
Plaque Assays.
HA Assay.
Hemadsorption.
One-Step Growth Curve.
Determination of Size.
Thermostability.
Sensitivity to Lipid Solvents.
Sensitivity to low pH.
Encephalomyocarditis Virus Neutralization.
Production of Interferon.
Plaque-Reduction Interferon Bioassay.
Inhibition of Cytopathic Effect (CPE) Interferon Bioassay.
Hemagglutination-Yield Reduction Bioassay.
Isolation of Viral Nucleic Acids from Virions.
Isolation of Viral Nucleic Acids from Infected Cells.
Analysis of Viral Nucleic Acids by Gel Electrophoresis.
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