Vaccinia Virus and Poxvirology

Vaccinia Virus and Poxvirology : Methods and Protocols

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Description

A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses - the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.show more

Product details

  • Hardback | 416 pages
  • 158 x 234 x 26mm | 839.16g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • New.
  • 96 black & white illustrations
  • 1588292290
  • 9781588292292

Back cover copy

Recombinant vaccinia viruses have become important in recent years, both as protein expression vectors, and-in the age of bioterrorism-as powerful tools for developing new smallpox vaccines, diagnostics, and understanding of poxvirus pathogenesis. In Vaccinia Virus and Poxvirology: Methods and Protocols, internationally recognized experts and leaders in poxvirology describe in step-by-step detail the successful techniques they have perfected to study and work with vaccinia virus and other poxviruses. They offer cutting-edge techniques for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxvirus-the latter critical to the important role of vaccinia virus in smallpox vaccination and its potential role as a vaccine vector directed against infectious agents and cancer. The protocols presented follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and timely, Vaccinia Virus and Poxvirology: Methods and Protocols offers virologists and vaccinologists an unequaled source of proven techniques for understanding poxviruses and developing defenses against their use as bioterrorist agents.show more

Review quote

From the foreword... "The right book at the right time." -Bernard Moss "...a collection of up-to-date protocols...the book is intended for virologists devoted to poxvirus research and scientists in vaccine research and some biotechnology area." - Chemotherapyshow more

Table of contents

Working Safely with Vaccinia Virus: Laboratory Technique and the Role of Vaccinia Vaccination Stuart N. Isaacs Construction and Isolation of Recombinant Vaccinia Virus Using Genetic Markers Maria M. Lorenzo, Inmaculada Galindo, and Rafael Blasco Construction of Recombinant Vaccinia Virus: Cloning into the Thymidine Kinase Locus Chelsea M. Byrd and Dennis E. Hruby Transient and Inducible Expression of Vaccinia/T7 Recombinant Viruses Mohamed Ragaa Mohamed and Edward G. Niles Construction of Recombinant Vaccinia Viruses Using Leporipoxvirus-Catalyzed Recombination and Reactivation of Orthopoxvirus DNA, Xiao-Dan Yao and David H. Evans Construction of cDNA Libraries in Vaccinia Virus Ernest S. Smith, Shuying Shi, and Maurice Zauderer Construction and Isolation of Recombinant MVA Caroline Staib, Ingo Drexler, and Gerd Sutter Growing Poxviruses and Determining Virus Titer Girish J. Kotwal and Melissa-Rose Abrahams Rapid Preparation of Vaccinia Virus DNA Template for Analysis and Cloning by PCR Rachel L. Roper Orthopoxvirus Diagnostics Hermann Meyer, Inger K. Damon, and Joseph J. Esposito An In Vitro Transcription System for Studying Vaccinia Virus Early Genes Steven S. Broyles and Marcia Kremer An In Vitro Transcription System for Studying Vaccinia Virus Late Genes Cynthia F. Wright Studying Vaccinia Virus RNA Processing In Vitro Paul D. Gershon Methods for Analysis of Poxvirus DNA Replication Paula Traktman and Kathleen Boyle Studying the Binding and Entry of the Intracellular and Extracellular Enveloped Forms of Vaccinia Virus Mansun Law and Geoffrey L. Smith Pox, Dyes, and Videotape: Making Movies of GFP-Labeled Vaccinia Virus Brian M. Ward Interaction Analysis of Viral Cytokine-Binding Proteins Using Surface Plasmon Resonance Bruce T. Seet and Grant McFadden Monitoring of Human Immunological Responses to Vaccinia Virus Richard Harrop, Matthew G. Ryan, Hana Golding, Irina Redchenko, and Miles W. Carroll Vaccinia Virus as a Tool for Immunologic Studies Nia Tatsis, Gomathinayagam Sinnathamby, and Laurence C. Eisenlohr Mouse Models for Studying Orthopoxvirus Respiratory Infections Jill Schriewer, R. Mark L. Buller, and Gelita Owens Viral Glycoprotein-Mediated Cell Fusion Assays Using Vaccinia Virus Vectors Katharine N. Bossart and Christopher C. Broder Use of Dual-Recombinant Vaccinia Virus Vectors to Assay Viral Glycoprotein-Mediated Fusion with Transfection-Resistant Primary Cell Targets Yanjie Yi, Anjali Singh, Joanne Cutilli, and Ronald G. Collman Poxvirus Bioinformatics Chris Upton Preparation and Use of Molluscum Contagiosum Virus from Human Tissue Biopsy Specimens Nadja V. Melquiot and Joachim J. Bugert Indexshow more

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