Vaccine Protocols

Vaccine Protocols : Second Edition

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Vaccine research and development is advancing at an unprecedented pace, with an increasing emphasis on rational design based upon a fundamental und- standing of the underlying molecular mechanisms. The aim of this volume is to provide a selection of contemporary protocols that will be useful to both novice and advanced practitioner alike. The variety of procedures required to design, develop, produce, and assess a vaccine is immense and covers aspects of ch- istry, biochemistry, molecular biology, cell biology, and immunology. No single volume can hope to cover these topics exclusively. Rather, here we attempt to provide a methods sourcebook focusing on hands-on practical advice. Comp- mentary and background information may be found in other volumes in the Me- ods in Molecular Medicine series. Of particular interest are volumes on Dendritic Cell Protocols, Interleukin Protocols, Vaccine Adjuvants, and DNA Vaccines. Since the publication of the first edition of Vaccine Protocols there have been major advances, particularly in the areas of bacterial genomics, antig- specific T-cell quantification, genetic manipulation of vaccine vectors, the h- nessing of natural molecules concerned with the regulation of immune responses, and the burgeoning field of DNA vaccinology. Hence, the extensive revision of this edition with new chapters on live viral vaccine vectors, atte- ated bacterial vectors, immunomodulators, MHC-peptide tetrameric complexes, and the identification of vaccine candidates by genomic analysis. Additionally, chapters from the first edition have been updated to accommodate state-of-t- art methods in vaccinology.
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Product details

  • Hardback | 416 pages
  • 158.5 x 237.2 x 31.2mm | 870.91g
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2003
  • XII, 416 p.
  • 1588291405
  • 9781588291400

Back cover copy

This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.
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Table of contents

Overview of Vaccines
Gordon Ada

Temperature-Sensitive Mutant Vaccines
Craig R. Pringle

Live Viral Vectors: Construction of a Replication-Deficient Recombinant Adenovirus
Anthony R. Fooks

Live Viral Vectors: Vaccinia Virus
Caroline Staib and Gerd Sutter

Live Viral Vectors: Semliki Forest Virus
Gunilla B. Karlsson and Peter J. Liljestroem

Development of Attenuated Salmonella Strains That Express Heterologous Antigens
Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londono-Arcila, and Gordon Dougan

Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria
Mark A. Reuter, Sean Hanniffy, and Jerry M. Wells

Synthetic Peptides
Michael J. Francis

Genetic Detoxification of Bacterial Toxins
Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, and Rino Rappuoli

Preparation of Polysaccharide-Conjugate Vaccines
Carla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret, Karen M. Reddin, and Jan T. Poolman

Adjuvant Formulations for Experimental Vaccines
Duncan E. S. Stewart-Tull

Incorporation of Immunomodulators into Plasmid DNA Vaccines
Linda S. Klavinskis, Philip Hobson, and Andrew Woods

Microencapsulation of Vaccine Antigens
David H. Jones

Lyophilization of Vaccines: Current Trends
Gerald D. J. Adams

Stimulation of Mucosal Immunity
David J. M. Lewis and Christopher M. M. Hayward

Induction and Detection of T-Cell Responses
Kingston H. G. Mills

Construction of MHC Class I-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses
Rachel V. Samuel and Tomas Hanke

Assessment of Functional Antibody Responses
Ray Borrow and Paul Balmer

The Use of Complete Genome Sequences in VaccineDesign
Nigel J. Saunders and Sarah Butcher

Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment
Michael J. Dennis

Clinical Trials
Paddy Farrington and Elizabeth Miller

Assuring the Quality and Safety of Vaccines: Regulatory Expectations for Licensing and Batch Release
Elwyn Griffiths and Ivana Knezevic

DNA Vaccination: An Update
Douglas B. Lowrie

From Vaccine Research to Manufacture: A Guide for the Researcher
Nigel Allison and Howard S. Tranter

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Review quote

"This manual serves as a valuable guide to the process of designing, producing and assessing vaccines used against infectious agents including viruses, bacteria and parasites. This book would most benefit scientists involved in the research and development associated with vaccine technology. It also would be most useful to those new to the field of vaccine formulation and production. Health and science libraries supporting research institutions, government agencies, academic and corporate entities involved in developing or producing vaccines will find this a valuable addition to the collection." -E-STREAMS

From reviews of the first edition...

"...cover[s] concepts and protocols applicable to a wide range of vaccines and infectious diseases and cancer...brings together many protocols of value to research and development scientists involved in the vaccine technology area."

-Immunology and Cell Biology

"...a valuable basic reference manual of vaccine related laboratory procedures...strongest in the area of vaccine development and delivery technology...especially valuable to those new to the field of vaccine development and formulation."

-Doody's Health Sciences Book Review Journal
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