Transgenic Animal Technology

Transgenic Animal Technology : A Laboratory Handbook

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Description

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals - from molecular methods to whole animal considerations - for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. Features: * Contains a comprehensive collection of transgenic animals and gene transfer methods * Discusses background and introduction to techniques and animal systems * Teaches practical step-by-step protocols * Describes mouse model systems * Covers laboratory and domestic animal species * Illustrated with diagrams and photographs * Includes useful tables and charts
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Product details

  • Spiral bound | 364 pages
  • 148.6 x 214.1 x 18.5mm | 358.33g
  • Academic Press Inc
  • San Diego, United States
  • English
  • illustrations, tables, charts
  • 0125571658
  • 9780125571654

Table of contents

Overview: Introduction to Transgenic Animals. Transgenic Animal Production Focusing on the Mouse Model: Transgenic Animal Production Using DNA Microinjection. Factors Affecting Transgenic Animal Production. Gene Transfer in Embryonic Stem Cells. Retrovirus-Mediated Gene Transfer. Gene Transfer Technology: Alternative Techniques and Applications. Molecular Approaches Involved in Mammalian Gene Transfer: Analysis of Transgene Integration. Production of Transgenic Laboratory and Domestic Animal Species: Production of Transgenic Rabbits and Rats. Production of Transgenic Poultry and Fish. Production of Transgenic Swine. Production of Transgenic Ruminants.
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Review quote

"First and foremost, this is a book for "everyone" interested in technology. The contributing authors have gone to great lengths to make the methodology clear and understandable to anyone with a working knowledge of molecularbiology and embryology. Each chapter is well-illustrated with diagrams and photographs and is accompanied by an extensive bibliography. Potential problems and possible solutions are discussed in detail. The second unique aspect of this book is its state of the art coverage of transgenesis in vertebrate animal species other than mice... Each chapter is well integrated with the others and is sufficiently detailed to be useful. The editor and contributing authors are to be congratulated for putting this information together in an easy accessible format which is sure to stimulate further interest and development in the field."--THERIOGENOLOGY"Transgenic Animal Technology is a laboratory manual that documents detailed methods required toaccomplish the gene transfers necessary to produce animals with altered genetic characteristics."--JOURNAL OF THE AMERICAN VETERINARY ASSOCIATION
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