Three-dimensional Electron Microscopy of Macromolecular Assemblies

Three-dimensional Electron Microscopy of Macromolecular Assemblies

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Three-Dimensional Electronic Microscopy of Macromolecular Assemblies is the first systematic introduction to single particle methods for reconstruction. It covers correlation alignment, classification, 3-D reconstruction, restoration, and interpretation of the resulting 3-D images oif macromolecular assemblies. It will be an indispensable resource for newcomers to the field and for all using or adopting these methods. Features: * Presents methods that offer an alternative to crystallographic techniques for molecules that cannot be crystallized * Describes methods that have been instrumental in exploring the three-dimensional structure of the nuclear pore complex, the calcium release channel,the ribosome, Chaperonins.
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Product details

  • Hardback | 330 pages
  • 154 x 230 x 20mm | 719.99g
  • Academic Press Inc
  • San Diego, United States
  • English
  • illustrations, bibliography, index
  • 0122650409
  • 9780122650406

Table of contents

Electron microscopy of macromolecular assemblies; two-dimensional averaging techniques; multivariate statistical analysis and classification of images; three-dimensional reconstruction; interpretation of three-dimensional images of macromolecules; example of an application - calcium release channel. Appendices: software implementations; macromolecular assemblies reconstructed from micrographs of single macromolecules.
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