Techniques for Mycorrhizal Research

Techniques for Mycorrhizal Research

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The past decade has seen not only an explosive increase of interest in mycorrhiza as evidenced by the surge of papers published, but also, the development of a greater awareness amongst plant physiologists, ecologists, and microbiologists that the mycorrhizal condition is the norm rather than the exception. Through this same period, new technologies (eg. NMR, RFLP, DNA mediated transformation, immunocytochemistry), which are readily applicable to mycorrhizal research, have become widely available. This two volume text presents an up-to-date collection of laboratory techniques that can be routinely used in mycorrhizal research. The first volume emphasizes a description of techniques applicable to ectomycorrhizal and ericoid systems, while the second stresses vesicular-arbuscular systems. Each contains a mix of papers, written by experts, which emphasize techniques, but which also include reviews of particular topics such as carbon metabolism, nitrogen metabolism, and applied aspects. The methods whereby, and the extent to which, mychorrhizal inoculation can be used to facilitate revegetation, as well as increases of agricultural or forest productivity are also described.
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Product details

  • Spiral bound | 896 pages
  • 171 x 235 x 51mm | 1,320g
  • Academic Press Inc
  • San Diego, United States
  • English
  • Reprint
  • 0125214901
  • 9780125214902

Table of contents

Introduction: State of the Art, J.L. Harley; Characterisation of Ectomycorrhiza, R. Agerer; Techniques in Synthesising Ectomycorrhiza, R.L. Peterson and P Chakravarty; Histochemistry of Ectomycorrhiza, R.L. Peterson; Nuclear Magnetic Resonance Studies in Ectomycorrhizal Fungi, F.M. Martin; Carbon Metabolism in Mycorrhiza, I. Jakobsen; Enzymology of Nitrogen Assimilation in Mycorrhiza, I. Ahmad and J.A. Hellebust; Techniques for the Study of Nitrogen Metabolism in Ectomycorrhiza, B. Botton and M. Chalot; Ectomycorrhizal DNA: Isolation RFLPs and Probe Hybridisation, P.T. Rygiewicz and J.L. Armstrong; Procedures and Prospects for DNA-Mediated Transformation of Ectomycorrhizal Fungi, F.A. Lemke, V. Barrett and R.K. Dixon; Principles of Use of Radiosotopes in Mycorrhizal Studies, P.B. Tinker, M.D. Jones and D.M. Durall; Techniques for Studying the Functional Aspects of Rhizomorphs of Woodrotting Fungi: Some Possible Applications to Ectomycorrhiza, C. H. Jennings; Structural and Ontogenic Study of Ectomycorrhizal Rhizomorphs, J.W.G. Cairney; Fine-Structural Analysis of Mycorrhizal Fungi and Root Systems: Negative Staining and Cryoelectron Microscopic Techniques, F. Mayer, M. Madkour, A. Nolte, and A. Varma; Epifluorescent Microscopy for Identification of Ectomycorrhiza, P. Cudlin; Electron Energy Loss Spectroscopy and Imaging Techniques for Subcellular Localisation of Elements in Mycorrhiza, J. Kottke; Methods for Studying Nursery and Field Response of Trees to Specific Ectomycorrhiza, D.H. Marx, J.L. Ruehle and C.E. Cordell; Maintenance of Ectomycorrhizal Fungi, H. Heinonen-Tansi and T. Holopainen; Root Window Technique for in Vivo Observation of Ectomycorrhiza on Forest Trees, S. Egii and J. Kalin; Experiments with Ericoid Mycorrhiza, J.R. Leake D.J. Read. Index.
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Review quote

A Review of the Original Volumes
"Mycorrhizal research has grown by leaps and bounds in the last recent years there has been an urgent need for a comprehensive and up-to-date source book of techniques; an easily available, detailed account of moderen, sophisticated methods routinely used in laboratories involved in mycorrhizal research. The present volumes meet this requirement well ... I applaud the editors for assembling an impeccable team of researchers, all of whom have contributed to the success of this volume which clearl provides more than mere laboratory procedures. Each author has attempted to provide a framework for the current status to his or her individual area, and outlined new and innovative methods along with the usage and significance of these methods. A vast amount of useful data generated from the application of the techniques reported is also shared. I see this volume as a tremendously valuable collection of methods in mycorrhizal research, seriously presented and snthesized ... This compilation is the best available."
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