Techniques for Microbiology

Techniques for Microbiology : A Student Handbook

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Description

Lammert's approach is visual and incorporates "voice balloons" that keep the student focused on the process described. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the American Society for Microbiology in its recommendations for the Microbiology Laboratory Core Curriculum (recommendations in which the author participated).
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Product details

  • Spiral bound | 240 pages
  • 156 x 226 x 8mm | 278g
  • Pearson
  • Upper Saddle River, NJ, United States
  • English
  • 0132240114
  • 9780132240116
  • 512,020

Back cover copy

This vivid, full-color laboratory techniques handbook is an instructive, concise, graphical presentation of the skills and techniques required in an introductory microbiology lab. Clear visual instructions enable readers to carry out fundamental manipulations and procedures effectively and safely. Demonstrates those techniques that will be used frequently for studying microbes in the laboratory. Has a safety section and frequent safety cautions throughout. Has a convenient, portable 6" x 9" trim size, a spiral binding and soft cover, making it ideal for use on the lab bench surface. It is priced inexpensively so that it will be suitable as a supplement to an in-house or commercial manual. Companion to any introductory laboratory whether for biology majors or allied health majors.
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Table of contents

Unit I - Safety first!



Safety in the microbiology laboratory



Universal precautions



Unit II - Culturing bacteria and aseptic techniques



Preparing culture media



Sterilization

The autoclave

Dry heat



Aseptic transfer of bacteria

Adjusting the gas burner

Preparing bacterial cultures in broth tubes and on agar slants

Transfer colony from plate to broth and to agar slant



Isolation of bacteria

Preparing a pour plate

Preparing a streak plate

Preparing a spread plate



Characteristic features of bacterial growth in broth and on agar



Maintenance and storage of stock cultures



Culturing anaerobic bacteria



Unit III - Visualizing bacteria



Effective use and responsible care of the light microscope



Measuring microscopic cells



Preparing a hanging-drop slide



Preparing a bacterial smear



Preparing a simple stain



Preparing a Gram stain



Preparing an acid-fast stain



Preparing a negative stain



Preparing a capsule stain



Preparing an endospore stain



Unit IV - Enzyme-based tests for the identification of bacteria



Hydrolytic (digestive) enzymes

Starch hydrolysis

Casein hydrolysis

Gelatin hydrolysis

Lipid hydrolysis (tributyrin and spirit blue agar)

DNA hydrolysis

Utilization of carbohydrates

Fermentation of carbohydrates (Durham tube)

Methyl red test (mixed fermentation)

Voges-Proskauer test (butanediol fermentation)

Citrate utilization

Oxidation-fermentation (O-F) glucose test



Degradation of amino acids

Indole test

H2 S production

Phenylalanine deamination

Decarboxylase test



Respiration tests

Catalase test

Oxidase test

Nitrate reduction



Miscellaneous tests

Triple sugar iron (TSI) test

Urea hydrolysis

Litmus milk reactions

Motility assay

Coagulase production

Selective and/or differential media

Blood agar

Eosin methylene blue (EMB) agar

Mannitol-salt agar

MacConkey agar

Phenylethyl alcohol (PEA) agar



Unit V - Counting microbes



Direct microscopic counting with the Petroff-Hausser chamber

Preparing a standard plate count of bacteria

Using Turbidemetry to estimate cell density

Plaque assay for determining bacteriophage titer



Unit VI - Measuring effectiveness of antibacterial chemicals



Kirby-Bauer method for sensitivity of bacteria to antibacterial

medicines

Evaluating antibacterial chemicals: The disk-diffusion

method
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