Techniques for the Analysis of Complex Genomes

Techniques for the Analysis of Complex Genomes

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Description

Faced with the search for genes associated with human diseases and the prospect of mapping and sequencing the human genome and those of other organisms, scientists must employ new strategies designed to map and clone large regions of DNA. Written by internationally renowned scientists, and containing contributions from some of the leading laboratories in the world, Techniques for the Analysis of Complex Genomes describes genomic mapping by pulsed field gel electrophoresis, and provides a detailed account of the cloning of large fragments of DNA using yeast artificial chromosomes (YACs) and bacteriophage PI cloning systems.
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Product details

  • Paperback | 237 pages
  • 152 x 229 x 19.05mm | 400g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0120576201
  • 9780120576203

Table of contents

YAC Library Construction and Vectors.
Mapping Clones and Localization of Probes.
YACs as Probes for cDNA Identification (Cloning of Islands).
Homologous Recombination (Integration of Vectors, Neo Targetting).
Building YACs Host Stains and Stability.
Cloning Telomers. Olgio and Genome Mapping.
P1 Cloning.
Microdisection.
Telomer Structure and Overview.
Genome PFGE.
SDS Approach to Olgio and Genome Mapping.
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