Techniques in Immunocytochemistry: v. 1
An extremely successful series of practical books for cell biologists and pathologists using immunocytochemical techniques. Volumes contain clear descriptions of techniques, plus hints on how to overcome problems and pitfalls. The series is aimed at graduates entering research, senior technicians, and pathologists.
- Paperback | 306 pages
- 158.75 x 234.95 x 19.05mm | 521.63g
- 31 Mar 1987
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
- New edition
- New edition
Table of contents
Contributors. Foreword. Preface. Tissue Preparation Methods for Immunocytochemistry, P.Brandtzaeg. Applications of Double-label Immunofluorescence, K.B. Pryzwansky. The Unlabelled Antibody Peroxidase-Anti-Peroxidase Method (PAP), J. Burns. The Protein A-Gold (pAg) Technique-A Qualitative and Quantitative Approach for Antigen Localization on Thin Sections, J. Roth. Double Immunoenzymatic Labelling, D.Y. Mason and R.-E. Woolston. "Double Bridge" Techniques of Immunocytochemistry, L.L. Vacca. Light Microscopic Immunocytochemistry with Fixed, Unembedded Tissues, R. Grzanna. Methods for Locating and Identifying Antigens in Plant Tissues, R.B. Knox. The Use of Proteolytic Enzymes for Improved Localization of Tissue Antigens with Immunocytochemistry, J.C.W. Finley and P. Petrusz. Pre-embedding Staining for Immunocytochemistry at the Light Microscopic Level, R.M. Franklin. Post-embedding Unlabeled Antibody Techniques for Electron Microscopy, P. Ordronneau. Specific Immunocytochemical Localization of Neuropeptides: A Utopian Goal?, F. van Leeuwen. Subject Index.
"These volumes provide useful and detailed firsthand experience of tackling many of the problems which are frequently encountered in the employment of antibodies (both monoclonal and polyclonal) and lectins as immunocytochemical reagents at the light and electron microscopic level. I recommend this series of books as a valuable introduction for the novice immunocytochemist and, more particularly, as useful and often enlightening reading for the experienced immunocytochemist." --JOURNAL OF CLINICAL PATHOLOGY