Synthetic Biology, Part A: Volume 497

Synthetic Biology, Part A: Volume 497 : Methods for Part/Device Characterization and Chassis Engineering

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Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.
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Product details

  • Hardback | 714 pages
  • 149.86 x 231.14 x 43.18mm | 1,292.73g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 497th edition
  • 0123850754
  • 9780123850751
  • 2,130,115

Table of contents

Section I. Measuring and Engineering Central Dogma Processes

Sequence-specificity and Energy Landscapes of DNA-binding Molecules
Joshua R. Tietjen, Leslie J. Donato, Devesh Bhimisaria and Aseem Z. Ansari

Promoter Reliability in Modular Transcriptional Networks
Rajat Anand, Navneet Rai and Mukund Thattai

The Analysis of ChIP-seq Data
Wenxiu Ma and Wing Hung Wong

Using DNA Microarrays to Assay Part Function
Virgil A. Rhodius and Carol A. Gross

Orthogonal gene expression in Escherichia coli
Wenlin An and Jason Chin

Directed evolution of promoters and tandem gene arrays for customizing RNA synthesis rates and regulation
Keith E.J. Tyo, Elke Nevoigt and Gregory Stephanopoulos

Section II. Device and System Design, Optimization, and Debugging

Design and Connection of Robust Genetic Circuits
Ron Weiss

Engineering RNAi circuits
Yaakov Benenson

From SELEX to cell: Dual selections for synthetic riboswitches
Joy Sinha, Shana Topp, and Justin P. Gallivan

Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells
Jeffrey V. Wong, Guang Yao, Joseph R. Nevins and Lingchong You

De novo design and construction of an inducible gene expression system in mammalian cells
Maria Karlsson, Wilfried Weber and Martin Fussenegger

BioBuilding: Using banana-scented bacteria to teach synthetic biology
James Dixon and Natalie Kuldell

Section III. Device Measurement, Optimization, and Debugging

Use of Fluorescence Microscopy to Analyze Genetic Circuit Dynamics
Gurol Suel

Microfluidics for synthetic biology: From design to execution
M. S. Ferry, I. A. Razinkov, J. Hasty

Plate-based assays for light-regulated gene expression systems
Jeffrey J. Tabor

Spatiotemporal control of small GTPases with light using the LOV domain
Yi I. Wu, Xiaobo Wang, Li He, Denise Montell and Klaus M. Hahn

Light Control of Plasma Membrane Recruitment using the Phy-PIF system
Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner

Synthetic Physiology: Strategies for Adapting Tools from Nature for Genetically-Targeted Control of Fast Biological Processes
Ed Boyden

Section IV. Devices for Metabolic Engineering

Metabolic Pathway Flux Enhancement by Synthetic Protein Scaffolding
John E. Dueber and Weston R. Whitaker

A Synthetic Iterative Pathway for ketoacid elongation
C. R. Shen, J. C. Liao

Section V. Expanding Chasses

Synthetic Biology in Streptomyces bacteria
Marnix Medema, Rainer Breitling and Eriko Takano

Methods for Engineering Sulfate Reducing Bacteria of the Genus Desulfovibrio
Kimberly L. Keller, Judy D. Wall and Swapnil Chhabra

Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons
Paul R. Jaschke, Rafael G. Saer, Stephan Noll and J. Thomas Beatty

Synthetic Biology in Cyanobacteria: Engineering and Analyzing Novel Functions
Thorsten Heidorn, Daniel Camsund, Hsin-Ho Huang, Pia Lindberg, Paulo Oliveira, Karin Stensjoe, and Peter Lindblad

Developing a Synthetic Signal Transduction System in Plants
Kevin J. Morey, Mauricio S. Antunes, Kirk D. Albrecht, Tessa A. Bowen, Jared F. Troupe, Keira L. Havens and June I. Medford

Lentiviral Vectors to Study Stochastic Noise in Gene Expression

Kate Franz, Abhyudai Singh, and Leor S. Weinberger
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