Studies from the Rockefeller Institute for Medical Research Volume 36

Studies from the Rockefeller Institute for Medical Research Volume 36

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1921 edition. Excerpt: ...1913, 476. mg. of hirudin for about 20 cc. of blood. After stoppering, this was gently shaken for a few minutes. 15 cc. were then transferred to the dialyzing membrane. The latter had previously been soaking in Ringer's solution, after which the excess fluid was wiped off of both sides with filter paper, and it was then affixed to a suitable glass tube mounted in a clamp on a stand. The dialyzing membrane, containing the blood, was then immersed in 1 liter of Ringer's solution,7 until the surfaces of the blood and the Ringer's solution were at the same level. A 2 cc. sample was taken at this time for analysis from the residue of the 20 cc. of blood, and at hourly intervals from the dialyzing blood. Before each sampling the temperature of the outside fluid was observed and the blood was mixed as well as possible. After sampling, the dialyzing bag was adjusted so that the surfaces of the fluids would again be at the same level. The Lewis-Benedict method, as modified by Myers and Bailey,8 was used for the blood sugar estimations. In many of the experiments the system was kept at a low temperature by surrounding the jar with chopped ice and salt. In some, powdered thymol was added to both fluids. As controls, normal dog blood was used. This was obtained and treated in exactly the same manner as the diabetic blood, except that weighed amounts of anhydrous dextrose were added in order to get a sugar content approximating the particular diabetic blood which it was desired to control. These controls were often shaken longer than the diabetic in order to be sure of solution of the dextrose; some even in a shaking machine to accomplish this. The control experiment was usually performed on the day after the diabetic and, of course, the attempt was...show more

Product details

  • Paperback | 180 pages
  • 189 x 246 x 10mm | 331g
  • Rarebooksclub.com
  • United States
  • English
  • black & white illustrations
  • 1236748409
  • 9781236748409