Studies from the Otho S.A. Sprague Memorial Institute; Collected Reprints Volume . 9

Studies from the Otho S.A. Sprague Memorial Institute; Collected Reprints Volume . 9

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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1921 edition. Excerpt: ... a quarter equivalents remained, probably as alanine, in the histidine fraction and that a maximum of one-fifth of an equivalent passed into amyl alcohol, possibly as an amine. In short, then, over half of the introduced available nitrogen was consumed by the microorganisms. (We have assumed that Z-alanine did not serve as a food.) Leucine and Histidine.--The growth obtained in this case was slightly better than that obtained on the histidine-alanine medium. The histamine production was increased by 18.6 per cent which is almost identical with that obtained on the alanine-histidine medium. Most of the leucine nitrogen appeared in the histamine fraction which indicates that the leucine was also decarboxylated. Tryptophane and Histidine.--The growth in this medium was about as dense as that obtained in the peptone-histidine medium; but the histamine production was decreased, not increased. The 26.3 per cent of histamine obtained in this case is about 5 per cent less than that obtained in a medium containing histidine as the only amino-acid. We anticipated some trouble with our colorimetric process in this case; but we were relieved to find that tryptophane, or its decomposition products, did not interfere in any way with the color production. Glutamic Acid and Histidine.--The growth on this medium was slightly less than that on the tryptophane-histidine medium. The presence of glutamic acid decreases the histamine production by about 9 per cent. The amino nitrogen figures indicate that most of the glutamic acid nitrogen was still attached to a carboxylated molecule. Cystine and Histidine.--The growth on this medium was very meager. The presence of cystine decreases the histamine production by 29 per cent so that the formation of this amine is...
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Product details

  • Paperback | 158 pages
  • 189 x 246 x 9mm | 295g
  • Miami Fl, United States
  • English
  • black & white illustrations
  • 1236625056
  • 9781236625052