Studies; Reprints Volume 19

Studies; Reprints Volume 19

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1914 edition. Excerpt: ...a I per cent Henderson phosphate mixture was used in all experiments, excepting the leucocyte and aseptic kidney experiment. Toluene was used as antiseptic. The mixtures for each experiment were made up as follows: Kidney. 1. 20.0 grams tissue suspended in 200 cc. alanine solution. 2. 5.0 grams tissue suspended in 50 cc. alanine solution. 3. 5.0 grams tissue suspended in 50 cc. phosphate solution. 4. 5.0 grams tissue suspended in 50 cc. phosphate solution. Lit/er. 1. 40.0 grams tissue suspended in 200 cc. alanine solution. 2. 10.0 grams tissue suspended in 50 cc. alanine solution. 3. 10.0 grams tissue suspended in 50 cc. phosphate solution. 4. 10.0 grams tissue suspended in 50 cc. phosphate solution. 5 Lang: H0fmeister'.r Beitriige, v, p. 321, 1904. '' Bostock: Biochem. J0urn., vi, p. 48, 1912. 1 Levene and Meyer: this Journal, xi, p. 365, 1912. Leucocytes. Approximately equal portions of leucocytes were mixed with 1. 50 cc. alanine solution. 2. 50 cc. alanine solution. 3. 50 cc. phosphate solution. 4. 50 cc. phosphate solution. In the aseptic kidney experiment only two suspensions were prepared; each minced kidney was suspended in 50 cc. alanine solution. In each instance, suspensions 2 and 4 were coagulated immediately after mixing; 1 and 3 were set aside at 37 for from one to three weeks. Methods of Analysis.---The contents of each flask were brought to a boil, coagulated and then poured into an excess of absolute alcohol to which was added sutficient 5 per cent alcoholic zinc chloride solution to completely precipitate the protein. This was filtered and the precipitate washed and extracted with hot water, and the filtrate and washing distilled in vacuum to completely remove the alcohol, which was only accomplished by...show more

Product details

  • Paperback | 182 pages
  • 189 x 246 x 10mm | 336g
  • Rarebooksclub.com
  • United States
  • English
  • black & white illustrations
  • 1236910923
  • 9781236910929