Stem Cells and Small RNAs

Stem Cells and Small RNAs : From Molecular Basis to Clinical Trials

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Product details

  • Hardback | 416 pages
  • 152 x 229mm
  • Academic Press Inc
  • San Diego, United States
  • English
  • 012800651X
  • 9780128006511

Table of contents

Contents Part I: Small RNAs 1. Outline 2. Rational design and high efficiency of siRNAs and shRNAs Molecular structures of small RNAs Working mechanisms of sRNAs Essential factors of rational design Biological traits of sRNAs with high efficiency Gene regulation of endogenous sRNAs 3. Side-effects of siRNA and shRNA Off-targets Identification of off-target effect Immune stimulatory effects Toxic effects of sRNA 4. miRNAs regulating noncoding and coding mRNAs Working principles of miRNAs Regulation of miRNA expression Discovery of ALU-based miRNAs Function of ALU-based miRNAs miRNA drugs 5. Discovery and functions of endogenous short-hairpin RNAs Discovery of endogenous shRNAs in human Silencing models of endogenous shRNAs Physiological function of endogenous shRNA Difference between endogenous shRNAs and miRNAs Evolution of shRNAs 6. Transfection of siRNAs into floating cells or stem cells Current issues of delivery systems Liposome-based transfection reagents Polymer-based veihcal Virus -based transduction methods Peptide nanoparticals 7. Transdermal delivery of small RNAs in vivo Injection of Nude siRNAs Cholesterol-conjugated siRNAs Transdermal and Mucusal delivery Inhalation aerosols Stable Nucleic Acid Lipid Particle (SNALP) 8. sRNAs and stem cells sRNA-omics of stem cells Regulation of stem cell self-renewal by sRNAs sRNA-mediated modulation on stem cell differentiation 9. siRNA gene drugs RNAi-mediated validation of genes as drug targets Special traits of siRNA-based drugs Clinical trials of siRNA drugs Development of siRNA drugs in China Part II: Stem Cells 1. Oultines 2. Embryonic stem cells Derivation of human embryonic stem cell lines Phenotypic analysis of human ESCs Genetic and epigenetic analysis of human ESCs ESC self-renewal and differentiation RNAi in ESCs 3. Adult stem cells Isolation and identification of MSCs from different sources Large-scale expansion of MSCs Identification and characterization of MSCs Senescence of MSCs Immuno-modulation and homing of MSCs Genetically engineering MSCs 4. Induced pluripotent stem cells Concept of reprogramming Phenotypic analysis of human iPSCs Genetic and epigenetic traits of human iPSCs Molecular mechanisms of reprogramming Human iPSC-based disease model for drug discovery 5. Trans-determination of stem cells Conversion of MSCs to neural stem cells Differentiation of MSCs to Cardiac stem cells Conversion of MSCs to endothelial stem cells Hepatogenic differentiation of MSCs Transformation of MSCs to pancreatic stem cells 6. Induced hematopoietic stem cells Conversion of MSCs into HSCs Immunocytochemical analysis of human stem cells Analysis of genome-wide gene expression profiles In vitro colony-forming assay In vivo reconstitution of marrow functions Classical cytogenetics: karyotyping 7. Cancer stem cells Isolation and characterization of CSCs Enrichment and culture of CSCs Molecular basis of CSC resistance Migration of CSCs Signaling networks of CSCs 8. Molecular mechanisms of trans-determination Concept of transdetermination state Genetic and epigenetic analysis of transdetermination Difference between reprogramming, de-differentiating and transdetermining Signaling networks of transdetermination Prospective importance of transdetermination 9. Stem cell drugs in China Concept of stem cell drugs R&D of Stem cell drugs Basic facilities and conditions Clinical studies Regulation of stem cell drugs Part III three: Methods 1. Isolation, characterization and maintenance of MSCs 2. Approaches for derivation and expansion of HSCs 3. Activation of MSC transdetermination state 4. Bioinformatics of transdetermination Microarray approach to identify signaling pathways responsible for transdetermination 5. Approaches for enrichment, characterization, and maintenance of cancer stem cells 6. Technologies to generate iPS cells Genetic engneering mRNA inducing methods miRNAs Small molecules 6. Microarrays of small RNAs 7. Molecular sensors detecting small RNAs 8. Micro-analysis of stem cell biological trails 9. Gene-modified stem cells 10. Clinical grade generation of stem cells Part IV: Application 1. Outlines 2. siRNA-based de-pigmenting and brightening cosmetics 3. siRNA treatment in psoriases model 4. miRNA and heart disease 5. MSC-derived HSCs transplantation 6. MSC-derived RBCs for fabrication of blood 7. MSC-derived RBCs for generation of platelets 8. MSC-derived RBCs for production of CIK cells 9. MSC-derived neural stem cells for cerebral palsy 10. Anti-aging application of MSCs 11. MSC-derived pancreatic stem cells and diabetes
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