Stem Cells in Regenerative Medicine

Stem Cells in Regenerative Medicine

By (author) 

Free delivery worldwide

Available. Dispatched from the UK in 2 business days
When will my order arrive?

Description

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology (TM) series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications.show more

Product details

  • Hardback | 424 pages
  • 195.58 x 261.62 x 27.94mm | 1,020.58g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2009 ed.
  • 19 Tables, black and white; 75 Illustrations, black and white; XX, 424 p. 75 illus.
  • 1588297977
  • 9781588297976

Back cover copy

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology(TM) series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Comprehensive and easy-to-use, Stem Cells in Regenerative Medicine: Methods and Protocols is certain to contribute greatly to the definition of standardized procedures for the manipulation of somatic and embryonic stem cells in research and clinical applications.show more

Review quote

"Presently, early detection and treatment remain our best hope for the cure of this common disease. Dr. Klein has assembled an outstanding group of authors to contribute to Management of Prostate Cancer. Those who take the time to read it will have a much better understanding of the management of prostate cancer."-Foreword by Ralph A. Straffon, MD ., ."Klein's Management of Prostate Cancer provides a balanced and comprehensive review...the 20 chapters are well written and provide a balanced view of the issues in diagnosis and treatment. The book's best feature is that it presents both sides of the picture...Thompson and Foley present in a point-counterpoint style arguments that support or refute the effectiveness of screening, the clinical significance of detected disease, and the effectiveness of local treatment, as well as opposing views on cost and the quality of data from prospective trials...A second feature of this book is its practical approach to the management of prostate cancer and the complications of treatment...The third feature of this book is its emphasis on new horizons...Management of Prostate Cancer is an informative review, and most of the chapters should be readily understood by patients and physicians alike. The last two chapters, on the surgeon's and radiation oncologist's perspectives, would be valuable reading for any man with prostate cancer who must decide whether to undergo surgery or radiation therapy. The chapter on screening provides important insights for primary care physicians facing the problem of whether to recommend screening."-The New England Journal of Medicine "This book is a must read for any physiciandesiring a complete and up-to-date understanding of prostate cancer. All aspects of this variable, complex, and prevalent disease are discussed in the 20 chapters, with contributions from 43 clinicians involved in the diagnosis and treatment of prostate cancer...Outstanding discussions analyze risk factors and genetic markers...The Management of Prostate Cancer will provide the clinician with a current, complete snapshot of all issues involved in the diagnosis and management of this disease. It should be part of the library of all urologists. Pathologists, radiation oncologists, medical oncologists, surgical oncologists, and general surgeons will find this text very useful as a reference for the latest developments in this field."-Current Surgery "The book offers recent survey in the field of prostate cancer epidemiology, risk factor, molecular markers, diagnostic procedures (including prostate biopsy), transrectal ultrasonography, computer tomography, magnetic resonance, the techniques of radical prostatectomy, radiotherapy and hormone therapy. Moreover there are on clinical practice oriented chapters, as interpretation of pathological findings, perioperative care, life quality evaluation, posttherapeutical urological disorders and modes of sexual rehabilitation, as well as adequate instructions of surgeon and radiotherapeutist for the period following adequate treatment....This publication represents an outstanding work devoted to the current state-of-the-art of the diagnosis and treatment of cancer, based on molecular-biological technology. It gives a comprehensive view about new achievements in molecular oncology and about innovations in research of humancancers. ...The book can be fully recommended for medical oncologists, surgeons and gynaccologists engaged in cancer treatment as a guide in this new rapidly developing topic....The book offers an up-to-date information on prostate cancer diagnostics and treatment and can be fully recommended to all physicians, especially to urologists, radiotherapeutists and oncologists."- Neoplasma ., ."this book is a comprehensive and concise update of the current knowledge of prostatic cancer....includes up-to-date information on the evaluation and treatment as well as the molecular biology, epidemiology and pathology of prostate cancer. Surgical treatment, radiation therapy, hormonal therapy, gene therapy and other therapeutic modalities are emphasized. Issues of early detection, quality of life and complications after treatment, and patient counseling are also broadly discussed....The strength of this book lies in its well-balanced and concise review of current diagnostic and therapeutic methods. Most of the illustrations and tables are good, and the references are current....The often ignored topic of hormone refractory prostate cancer is well presented....this book is practical, well illustrated and of value for those who would like to have a better understanding of the management of prostate cancer. It is a worthwhile resource for the general urologist, radiation and urological oncologists as well as for residents in training." - Journal of Urology ., ."this book is both timely and useful for all clinicians who wish to obtain a clear and up-to-date account of the management of prostate cancer." - British Journal of Surgery ., ."interesting and well-written...particularly useful to understand the surgical management of prostate cancer." - Int. J. Radiation Oncology Biol. Physshow more

Table of contents

Preface Contributors Section I: Stem Cell Sources and Manipulation Derivation and Manipulation of Murine Embryonic Stem Cells Alexander Meissner, Sarah Eminli and Rudolf Jaenisch Patterning Mouse and Human Embryonic Stem Cells using Micro-Contact Printing Raheem Peerani, Celine Bauwens, Eugenia Kumacheva, and Peter W. Zandstra Efficient Gene Knockdowns in Human Embryonic Stem Cells using Lentiviral Based RNAi Asmin Tulpule and George Q. Daley Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells Aziza P. Manceur and Julie Audet Stem Cell Sources for Regenerative Medicine Ali M. Riazi, Sarah Y. Kwon and William L. Stanford Section II: Regeneration of the Blood System Investigating the Interactions between Hemopoietic Stem Cells and their Niche: Methods for the Analysis of Stem Cell Homing and Distribution within the Marrow following Transplantation Brenda Williams and Susan K. Nilsson Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells Valerie Cortin, Nicolas Pineault, and Alain Garnier Ex vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering Luc Douay and Marie-Catherine Giarratana Section III: Regeneration of the Nervous System Isolation and Manipulation of Mammalian Neural Stem Cells in Vitro Claudio Giachino, Onur Basak and Verdon Taylor Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors Karl J.L. Fernandesand Freda D. Miller Xenotransplantation of Embryonic Stem Cell-Derived Motor Neurons into the Developing Chick Spinal Cord Hynek Wichterle, Mirza Peljto and Stephane Nedelec Transplantation of Neural Stem/Progenitor Cells into Developing and Adult CNS Tong Zheng, Gregory P. Marshall II, K. Amy Chen, and Eric D. Laywell Transplantation of Embryonic Stem Cell-Derived Dopaminergic Neurons in MPTP-Treated Monkeys Jun Takahashi, Yasushi Takagi and Hidemoto Saiki Section IV: Regeneration of the Epidermis Isolation and Culture of Epithelial Stem Cells. Jonathan A. Nowak and Elaine Fuchs Regeneration of Skin and Cornea by Tissue Engineering Danielle Larouche, Claudie Paquet, Julie Fradette, Patrick Carrier, Francois A. Auger and Lucie Germain Section V: Regeneration of the Musculoskeletal System Prospective Isolation of Mesenchymal Stem Cells from Mouse Compact Bone Brenton J. Short, Nathalie Brouard and Paul J. Simmons Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs) Rahul Sarugaser, Jane Ennis, William L. Stanford and John E. Davies Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transduction and Conditioned Media Production Massimiliano Gnecchi and Luis G. Melo Template DNA Strand Co-segregation and Asymmetric Cell Division in Skeletal Muscle Stem Cells Vasily Shinin, Barbara Gayraud-Morel, and Shahragim Tajbakhsh Isolation and Grafting of Single Muscle Fibres Charlotte A. Collinsshow more