In Situ Hybridization for the Brain

In Situ Hybridization for the Brain

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In situ hybridization is a valuable probe technique of particular use to the neuroscientist as it allows identification of a specific cell type expressing a given gene from amont the many making up neuronal tissue. This book is for neurobiologists wishing to set up this method who want a simple straightforward protocol. It concentrates on the radiolabelled-oligonucleotide method in the first part of the book, with the second part directed to more recent, less standardized non-radioactive methods.
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Product details

  • Spiral bound | 300 pages
  • 197.4 x 254.8 x 13mm | 343.38g
  • Academic Press Inc
  • San Diego, United States
  • English
  • Illustrationssome col.)
  • 0127599207
  • 9780127599205

Table of contents

INCLUDE: Detailed Method of ISH . Processing Vertebrate Embryos and Early Postnatal Material for ISH . Logistics of Hybridizing/Processing Very Large Brain Sections E.G. Primates, Sheep . Logistics of Hybridizing Oligonucleotides to Human Postmortem Tissue . ISH on Cell Culture, Astrocytes and Neuronal . ISH with Organotypic Neuronal Cultures . Quantitation of ISH . 35soligonucleotides + Antibodies, Using Alternate Sections 35soligonucleotides + NADPH Diaphorase . 35sonucleotides + Antibodies, On the Same Sections . Non Radioactive in Situ Hybridization Using Chemically Modified Oligonucleotides . Combined Immunocytochemistry, ISH and Tract Tracing . Drosophila CNS Radioactive and Nonradioactive Approaches for ISH . Whole Mount ISH Approaches to the Vertebrate Retina
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