In Situ Hybridization

In Situ Hybridization : Applications to Neurobiology

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A technique for reading the messenger RNAs by which cells express their distinctive populations of structural and functional proteins, in situ hybridization is now reliable and practical for use in neurobiological research. This book is a guide to methods and applications for the working neuroscientist. The authors, all leaders in developing and refining the techniques under discussion, begin with a general account of in situ hybridization and eukaryotic gene expression, and go on to describe, in step-by-step detail, a number of specific research applications. Readership: Students, reseachers and laboratory workers concerned with the cell and molecular biology of the nervous system.
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Product details

  • Hardback | 256 pages
  • 160 x 240mm | 698g
  • New York, United States
  • English
  • 68 illustrations, 14 in colour
  • 0195048598
  • 9780195048599

Table of contents

F.E. Bloom: Foreword; L.H. Tecott, J.H. Eberwine, J.D. Barchas, & K.L. Valentino: Methodological considerations in the utilization of in situ hybridization; J.P. Coghlan, J.D. Penschow, J.R. Fraser, P. Aldred, J. Haralambidis, & G.W. Tregear: Gene expression in mammalian cells; L.A. Angerer, M.H. Stoler, & R.C. Angerer: In situ hybridization with RNA probes: an annotated recipe; R.H. Singer, J.B. Lawrence, & R.N. Rashtchian: Toward a rapid and convenient in situ methodology using isotopic and non-isotopic probes; W.S.T. Griffin: Methods for hybridization and quantitation of mRNA in individual brain cells; B.S. Schacter: Studies of neuropeptide gene expression in brain and pituitary; S. J. Watson, T.J. Sherman, J.E. Kelsey, S. Burke, H. Akil: Anatomical localization of mRNA: in situ hybridization of neuropeptide systems; G.A. Higgins & M.C. Wilson: In situ hybridization for mapping the neuroanatomical distribution of novel brain mRNAs; R.J. Milner, G.A. Higgins, H. Schmale, F.E. Bloom; J.E. Pintar, D.I. Lugo: Localization of peptide hormone gene expression in adult and embryonic tissue; A.T. Haase: Analysis of viral infections by in situ hybridization.
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