Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods: Volume 582
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Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods: Volume 582

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Description

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.
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Product details

  • Hardback | 484 pages
  • 152 x 229 x 26.92mm | 930g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0128093102
  • 9780128093108

Table of contents

How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp
J. Sung, K.I. Mortensen, J.A. Spudich and H. Flyvbjerg
Studying the Mechanochemistry of Processive Cytoskeletal Motors with an Optical Trap
V. Belyy and A. Yildiz
Single-Molecule Optical Trapping Techniques to Study Molecular Mechanisms of a Replisome
B. Sun and M.D. Wang
Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy
M. H. Shabestari, A.E.C. Meijering, W.H. Roos, G.J. L. Wuite and E.J.G. Peterman
Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping
C.-T. Lin and T. Ha
High-Resolution Optical Tweezers Combined with Single-Molecule Confocal Microscopy
K.D. Whitley, M.J. Comstock and Y.R. Chemla
Integrating Optical Tweezers, DNA Tightropes and Single Molecule Fluorescence Imaging: Pitfalls and Traps
J. Wang, J.T. Barnett1, M.R. Pollard and N.M. Kad
Single-Stranded DNA Curtains for Studying Homologous Recombination
C.J. Ma, J.B. Steinfeld and E.C. Greene
Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch Repair
M.W. Brown, A.D.L. Torre and I.J. Finkelstein
Single-Molecule Insight into Target Recognition by CRISPR-Cas Complexes
M. Rutkauskas, A. Krivoy, M. Szczelkun, C. Rouillon and R. Seidel
Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules
C. Duboc, J. Fan, E.T. Graves and T.R. Strick
Measuring Force Induced Dissociation Kinetics of Protein Complexes Using Single Molecule Atomic Force Microscopy
K. Manibog, C.-F. Yen and S. Sivasankar
Improved Force Spectroscopy Using Focused-Ion-Beam Modified Cantilevers
J.K. Faulk, D.T. Edwards, M.S. Bull and T.T. Perkins
Single-Molecule Characterization of DNA-Protein Interactions Using Nanopore Biosensors
A.H. Squires, T. Gilboa, C. Torfstein, N. Varongchayakul and A. Meller
Subangstrom Measurements of Enzyme Function using a Biological Nanopore, SPRNT
A.H. Laszlo, I.M. Derrrington and J.H. Gundlach
Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics
S. Ucuncuoglu, D.A. Schneider, E.R. Weeks, D. Dunlap and L. Finzi
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Review Text

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia " The Methods in Enzymology series represents the gold-standard." --Neuroscience
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Review quote

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
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About Yann R Chemla

Associate Professor of Biochemistry, Carver College of Medicine, University of Iowa, USA Associate Professor of Physics and Biophysics, Department of Physics, University of Illinois at Urbana-Champaign, USA
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