Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods: Volume 581
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Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods: Volume 581

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Description

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion.
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Product details

  • Hardback | 616 pages
  • 152 x 229 x 35.56mm | 1,130g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 012809267X
  • 9780128092675

Review quote

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
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About Maria Spies

Associate Professor of Biochemistry, Carver College of Medicine, University of Iowa, USA Associate Professor of Physics and Biophysics, Department of Physics, University of Illinois at Urbana-Champaign, USA
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Table of contents

Direct Fluorescent Imaging of Translocation and Unwinding by Individual DNA Helicases
T.L. Pavankumar, J.C. Exell and S.C. Kowalczykowski
Single Molecule Imaging with one Color Fluorescence
Y. Qiu and S. Myong
Measuring Membrane Protein Dimerization Equilibrium in Lipid Bilayers by Single-Molecule Fluorescence Microscopy
R. Chadda and J.L. Robertson
Fluorescent Labeling of Proteins in Whole Cell Extracts for Single-Molecule Imaging
S.R. Hansen, M.L. Rodgers and A.A. Hoskins
Quantifying the Assembly of Multicomponent Molecular Machines by Single-Molecule Total Internal Reflection Fluorescence Microscopy
E.M. Boehm, S. Subramanyam, M. Ghoneim, M.T. Washington and M. Spies
How to Measure Separations and Angles Between Intra-Molecular Fluorescent Markers
K.I. Mortensen, J. Sung, J.A. Spudich and H. Flyvbjerg
Precisely and Accurately Inferring Single-Molecule Rate Constants
C.D. Kinz-Thompson, N.A. Bailey, R.L. Gonzalez
Quantification of Functional Dynamics of Membrane Proteins Reconstituted in Nanodiscs Membranes by Single Turnover Functional Readout
M.E. Moses, P. Hedegard and N.S. Hatzakis
Putting Humpty-Dumpty Together: Clustering the Functional Dynamics of Single Biomolecular Machines Such as the Spliceosome
C.E. Rohlman, M.R. Blanco and N.G. Walter
Single Molecule FRET to Measure Conformational Dynamics of DNA Mimatch Repair Proteins
J.W. Gauer, S. LeBlanc, P. Hao, R. Qiu, B. Case, M. Sakato, M.M. Hingorani, D.A. Erie and K.R. Weninger
Single Molecule Confocal FRET Microscopy to Dissect Conformational Changes in the Catalytic Cycle of DNA Topoisomerases
S. Hartmann, D. Weidlich and D. Klostermeier
Probing the Conformational Landscape of DNA Polymerases Using Diffusion-Based Single-Molecule FRET
J. Hohlbein and A.N. Kapanidis
Methods for Investigating DNA Accessibility with Single Nucleosomes
M.D. Gibson, M. Brehove, Y. Luo, J. North and M.G. Poirier
Single-Molecule Fluorescence Studies of Fast Protein Folding
Z. Wang, L.A. Campos and V. Munoz
Single-Molecule Multi-Color FRET Assay for Studying Structural Dynamics of Biomolecules
S. Lee, Y. Jang, S.-J. Lee and S. Hohng
A Multi-Color Single Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously
M. Goetz, P. Wortmann, S. Schmid and T. Hugel
Interferometric Scattering Microscopy for the Study of Molecular Motors
J. Andrecka, Y. Takagi, K.J. Mickolajczyk, L.G. Lipert, J.R. Sellers, W.O. Hancock, Y.E. Goldman and P. Kukura
Enzyme Kinetics in Femtoliter Arrays
P. Mogalisetti and D.R. Walt
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Review Text

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." -- Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
show more