Scientific American Supplement Volume 54

Scientific American Supplement Volume 54

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1902 edition. Excerpt: ...hydroxid. In 1880 Nenki found that anaerobic bacteria grow well in an atmosphere from which the oxygen has been absorbed by an alkaline solution of pyrogallic acid. Practical application of this principle, however, was not made until 1888, when Buchner invented the method which now carries his name. Buchner's Method.--Use two test tubes of diiferent si_zes (see Fig. 30), drop into the large tube a small wire support on which the small tube is subsequently placed. Tha small test tube contains the inoculated medium and is closed with a loose cotton plug_ Put into the large test tube one gramme of dry pyrogallic acid and then ten cubic centimeters of a one-tenth solution of potassium hydroxid (1 part liquor kali caust. to 10 parts of water). Then quickly lower the small culture tube and close the latter hermetically with a new, elastic, well-fitting, parafiined rubber stopper. Shake well. Buchner observed that the above-stated amount of pyrogallic acid and potassium hydroxid will completely absorb the oxygen in a tube with a cubic content of 100 cubic centimeters in 24 hours at incu the desiccator hermetically with a vaselined ground glass plate the apparatus is put into the incubator. This method enables the experimenter to cultivate numerous tube cultures simultaneously and under identical conditions. The authors obtained the best results when they used media containing 2 per cent glucose. Blucher (1890) recommends the following method for plate cultures: Put a petri dish 6 centimeters in diameter, minus cover, into a glass saucer 10 centimeters in diameter and 4 centimeters high. The upper edge of the saucer is ground convexly and it fits exactly into a cover with a concave rim. Into the outer saucer pyrogallic acid and more

Product details

  • Paperback | 886 pages
  • 189 x 246 x 44mm | 1,551g
  • United States
  • English
  • black & white illustrations
  • 123690267X
  • 9781236902672