The Resolution Revolution: Recent Advances In cryoEM: Volume 579

The Resolution Revolution: Recent Advances In cryoEM: Volume 579

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Description

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.
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Product details

  • Hardback | 486 pages
  • 152 x 229 x 27.94mm | 910g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0128053828
  • 9780128053829

Review quote

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
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About R. Crowther

Tony Crowther is an emeritus scientist at the MRC Laboratory of Molecular Biology in Cambridge, where he was joint head of the Structural Studies Division from 1994 to 2005. He has been involved in electron microscopy and image processing of biological structures for many years. His particular biological interests have been in viruses such as hepatitis B and in the abnormal filaments that form in neurodegenerative diseases. He is a Fellow of the Royal Society of London, a Fellow of the Academy of Medical Sciences and a member of the European Molecular Biology Organization.
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Table of contents

Direct Electron Detectors - Methods in Enzymology (2016)
Greg McMullan, Abdul R. Faruqi and Richard Henderson
Specimen Behavior in the Electron Beam
Robert M. Glaeser
Specimen Preparation for High-Resolution Cryo-EM
Christopher J. Russo and Lori A. Passmore
Strategies for Automated Cryoem Data Collection Using Direct Detectors
Anchi Cheng, Yong Zi Tan, Venkat Dandey, Clinton S. Potter and Bridget Carragher
Processing of Cryo-EM Movie Data
Zev A. Ripstein and John L. Rubinstein
Processing of Structurally Heterogeneous Cryo-EM Data in RELION
Sjors H.W. Scheres
Single Particle Refinement and Variability Analysis in EMAN2.1
Steven J. Ludtke
Frealign: An Exploratory Tool for Single Particle Cryo-EM
Nikolaus Grigorieff
Testing the Validity of Single Particle Maps at Low and High Resolution
Peter B. Rosenthal
Tools for Model Building and Optimization into Near Atomic Resolution Electron Cryo-Microscopy Density Maps
Frank DiMaio and Wah Chiu
Refinement of Atomic Structures Against CryoEM Maps
Garib N. Murshudov
Cryo-EM Structure Determination Using Segmented Helical Image Reconstruction
Simon A. Fromm and Carsten Sachse
Cryo-Electron Tomography and Subtomogram Averaging
William Wan and John A.G. Briggs
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Review Text

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience
show more