Renal Disease

Renal Disease : Techniques and Protocols

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"Rule IV. There is need of a method for finding out the truth. Rule V. Method consists entirely in the order and disposition of the objects toward which our mental vision must be directed if we would find out any truth. We shall comply with it exactly if we reduce involved and obscure propositions step be step to those that are s- pler, and then starting with the intuitive apprehension of all those that are absolutely simple, attempt to ascend to the knowledge of all others by precisely similar steps. " -Rene Descartes, Rules for the Direction of Mind "...Perhaps he would sooner satisfy himself by resolving light into colours as far as may be done by Art, and then by examining the properties of those colours apart, and afterwards by trying the effects of reconjoyning two or more or all of those, and lastly by separating them again to examine what changes that reconjunction had wrought in them. This will prove a tedious and difficult task to do it as it ought to be done but I could not be satisfied till I had gone through it. " -From Newton's letter, quoted in The Life of Isaac Newton by Richard Westfall. Cambridge University Press, 1993.show more

Product details

  • Hardback | 500 pages
  • 154 x 228 x 30mm | 821.02g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2003 ed.
  • XVIII, 500 p.
  • 1588291340
  • 9781588291349

Back cover copy

Today's investigator, overwhelmed by the information avalanche, may find it difficult to analyze emerging advances in technology. In Renal Disease: Techniques and Protocols, leading experts distill their experience into user-friendly methods for studying fluid-electrolyte homeostasis and kidney function in health and disease. Described in step-by-step detail, the techniques move from the molecular level to the whole organism, and from simple models to integrative physiology. Here the researcher will find established disease models, methods for optimizing renal disease research, as well as much help in choosing imaging techniques for studies of the kidney's structure and function in health, disease, and during embryonic development. Subjects covered include molecular diagnostics, strategies for studying the molecular mechanisms of kidney disease, technical means to assess the functional correlates of disease, and the planning of clinical trials. In addition to detailed instructions, each cutting-edge method includes troubleshooting advice, lists of reagents, and tips on avoiding pitfalls. State-of-the-art and highly practical, Renal Disease: Techniques and Protocols is not only a vade mecum for the many techniques employed to study the kidney in health and disease today, but also a user-friendly guide for the uninitiated, and a thoughtful consult for the experienced researcher.show more

Table of contents

Part I. Optimizing the Usage of Models of Renal Disease Standards of Animal Care in Biological Experiments Ellen M. Levee Models of Polycystic Kidney Disease Poornima Upadhya Rat Models of the Metabolic Syndrome Stevan P. Tofovic and Edwin K. Jackson Models of Glomerulonephritis Raghu V. Durvasula and Stuart J. Shankland Part II. Choices of Imaging Techniques in Studies of Renal Disease Functional Studies of the Kidney with Magnetic Resonance Imaging Hani B. Marcos, Yantian Zhang, and Peter L. Choyke Use of Radionuclides to Study Renal Function Yiyan Liu and M. Donald Blaufox Intravital Videomicroscopy Tokunori Yamamoto and Fumihiko Kajiya Confocal and Two-Photon Microscopy Janos Peti-Peterdi and P. Darwin Bell Atomic Force Microscopy in Renal Physiology Robert M. Henderson Freeze-Fracture Analysis of Renal-Epithelial Tight Junctions Hiroyuki Sasaki Part III. Studies of Renal Development Organ Culture of Intact Metanephric Kidneys Simon J. M. Welham and Adrian S. Woolf The Ureteric Bud: Tissue-Culture Approaches to Branching Morphogenesis and Inductive Signaling Alan O. Perantoni Studies of Cell Lineage in the Developing Kidney Maria Luisa S. Sequeira Lopez and R. Ariel Gomez Transient Transfection Assays for Analysis of Signal Transduction in Renal Cells Robin L. Maser, Brenda S. Magenheimer, Christopher A. Zien, and James P. Calvet Part IV. Approaches to Study Molecular Mechanisms of Disease The Study of Gene Polymorphisms: How Complex Is Complex Genetic Disease? Sylvia Bahring, Atakan Aydin, and Friedrich C. Luft Laser-Capture Microdissection Laura Barisoni and Robert A. Star Serial Analysis of Gene Expression M. Ashraf El-Meanawy, Shrinath Barathan, Patrick S.Hayden, Sudha K. Iyengar, Jeffrey R. Schelling, and John R. Sedor RNA Labeling and Hybridization of DNA Microarrays Erwin P. Bottinger, Akiva Novetsky, and Jiri Zavadil Gene-Expression Analysis of Microdissected Renal Biopsies Clemens D. Cohen and Matthias Kretzler The Use of SELDI ProteinChip (R) Array Technology in Renal Disease Research Eric Fung, Deb Diamond, Anja Hviid Simonsesn, and Scot R. Weinberger Part V. Technical Means to Assess Functional Correlates of Disease Clearance Studies in Genetically Altered Mice William T. Noonan and John N. Lorenz Long-Term Blood-Pressure Monitoring in Unrestrained Animals Karen A. Griffin, Isam Abu-Amarah, and Anil K. Bidani In Vitro Studies on Renin Release Boye L. Jensen, Ulla G. Friis, and Ole Skott Mitochondrial Function Joel M. Weinberg and Pothana Saikumar Detection of Cysteine S-Nitrosylation and Tyrosine 3-Nitration in Kidney Proteins Mark Crabtree, Gang Hao, and Steven S. Gross Products of Arachidonic Acid Metabolism Mairead A. Carroll, John C. McGiff, and Nicholas R. Ferreri Methods for Measurements of Heme Oxygenase (HO) Isoforms-Mediated Synthesis of Carbon Monoxide and HO-1 and HO-2 Proteins Nader G. Abraham, Houli Jiang, Michael Balazy, and Alvin I. Goodman The Juxtamedullary Nephron Preparation Daniel Casellas and Leon C. Moore Tubuloglomerular Feedback Volker Vallon and Jurgen Schnermann Microdissected Perfused Vessels Thomas L. Pallone Renal Microperfusion Techniques Charles S. Wingo, I. David Weiner, and Shen-Ling Xia How to Design a Clinical Trial Bryan M. Curtis, Brendan J. Barrett, and Patrick S. Parfrey Conclusion Michael S. Goligorsky Indexshow more