Recombinant DNA Methodology II

Recombinant DNA Methodology II

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Description

This volume contains a selection of articles from volumes 216, 217 and 218 of "Methods in Enzymology". Key features include methods for: DNA isolation and cloning; synthesizing complementary DNA (cDNA); cleaving and manipulating DNA; selecting useful reporter genes; constructing vectors for cloning genes; constructing expression vectors; site-directed mutagenesis and gene disruption; identifying and mapping genes; transforming animal and plant cells; sequencing DNA; amplifying and manipulating DNA by PCR; and detecting DNA-protein interaction.
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Product details

  • Hardback | 935 pages
  • 183.9 x 243.33 x 35.31mm | 1,442.42g
  • Academic Press Inc
  • San Diego, United States
  • English
  • index
  • 0127655611
  • 9780127655611

Back cover copy

Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels.
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Table of contents

Isolation, synthesis and detection of DNA and RNA; enzymes and methods for cleaving and manipulating DNA; reporter genes; vectors for cloning genes; vectors for expressing cloned genes; mutagenesis and gene disruption; screening libraries, identifying and mapping genes; methods for transforming animal and plant cells; methods for sequencing DNA; polymerase chain reaction for amplifying and manipulating DNA; methods for detecting DNA-protein interaction; other methods.
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