Recombinant DNA Methodology II
This volume contains a selection of articles from volumes 216, 217 and 218 of "Methods in Enzymology". Key features include methods for: DNA isolation and cloning; synthesizing complementary DNA (cDNA); cleaving and manipulating DNA; selecting useful reporter genes; constructing vectors for cloning genes; constructing expression vectors; site-directed mutagenesis and gene disruption; identifying and mapping genes; transforming animal and plant cells; sequencing DNA; amplifying and manipulating DNA by PCR; and detecting DNA-protein interaction.
- Hardback | 935 pages
- 183.9 x 243.33 x 35.31mm | 1,442.42g
- 01 Oct 1995
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Back cover copy
Recombinant DNA methods are powerful, revolutionary techniques that allow the isolation of single genes in large amounts from a pool of thousands or millions of genes and the modification of these isolated genes or their regulatory regions for reintroduction into cells for expression at the RNA or protein levels.
Table of contents
Isolation, synthesis and detection of DNA and RNA; enzymes and methods for cleaving and manipulating DNA; reporter genes; vectors for cloning genes; vectors for expressing cloned genes; mutagenesis and gene disruption; screening libraries, identifying and mapping genes; methods for transforming animal and plant cells; methods for sequencing DNA; polymerase chain reaction for amplifying and manipulating DNA; methods for detecting DNA-protein interaction; other methods.