Recombinant Antibodies

Recombinant Antibodies : Applications in Plant Science and Plant Pathology

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Description

The first part of this book combines basic information on antigens and antibodies with a review of technologies for both the development of recombinant antibodies and their application. The principles involved in producing recombinant antibodies from either pre-existing monoclonal antibodies or from phage display libraries are introduced with practical examples. Modern methods for the analysis of antibody binding properties are described in detail. Examples are given in the second part of this book for the application of recombinant antibody technology to antibody-mediated resistance to plant disease and immunomodulation of plant antigens. The potential of antibody-expressing plants as bioreactors for large-scale production and storage of recombinant antibodies is described and a future outlook given.show more

Product details

  • Hardback | 216 pages
  • 170.2 x 246.4 x 12.7mm | 476.28g
  • Taylor & Francis Ltd
  • London, United Kingdom
  • English
  • 46 b&w illustrations
  • 0748407634
  • 9780748407637

Table of contents

Part 1 Recombinant Antibodies as Diagnostic Tools in Plant Pathology1. Antigens, Antibodies and Plantibodies Pascale Seddas-Dozolme, BVernard Walter and Marc H.V. Van Regenmortel 2. Cloning of Pre-existing Hybridomas - Production of Novel Diagnostic Tools for Plant Pathology Angelika Ziegler 3.Selection of Anti-plantVirus Single Chain Variable Fragments From Phage Display Antibody Libraries Karen Harper, Rachel L. Toth, Mike A. Mayo and Lesley Torrance 4.Dedicated Expression Vectors for the Production of Diagnostic Reagents Randolf J. Kerschbaumer and GottfriedHimmler 5.Analysis of Antibody Binding Properties Gabrielle Zeder-Lutz and Marc H.V. Van Regenmortel 6.Antibodies as Diagnostic Tools in Plant Pathology Lesley Torrance PART 2 Antibodies in Plant Biotechnology7.Production of Antibodies in Transgenic Plants-a General Introduction Chris de Wilde, Geert de Jaeger, Myriam de Neve, Marc Van Montagu and Ann Depicker 8.Transgenic Plants-a Low Cost Production System For Recombinant Antibodies Ulrike Fiedler, OlgaArtsaenko, Julian Phillips, and Udo Conrad 9.Intracellular Immunomodulation in Plants-a New Tool for the Investigation of Phytohormones Olga Artsaenko, Julian Phillips, Ulrike Fiedler, Martin Peisker, and Udo Conrad 10.Engineering of Beet Necrotic YellowVein Virus (BNYVV) Resistance in Nicotiana Benthamiana Lothar F. Fecker and Renate Koenig 11.Engineering Antibody Mediated Resistance to Plant Root Nematodes - Potential and Prospects Geert Smant, Jaap Bakker, Fred J. Gommers and Arjen Schotts12.Antibodies in Plant Biotechnology AngelikaZiegler and Karen Harper Bakker, Department of Nematology, The Netherlands, U. Conrad, Institut fur Pflankzengenetik und Kulturpflanzenforschung Gatersleben, Germany, G. De Jaeger, University of Gent, Belgium,M. De Neve, University of Gent, Belgium, A. Depicker, University of Gent, Belgium, C. De Wilde, University of Gent, Belgium, L. Fecker, Institut fur Pflankzengenetik und Kulturpflanzenforschung Gatersleben, Germany, U. Fiedler, Martin Luther UniversitatHalle-Wittenberg, F. Gommers, Department of Nematology, The Netherlands, K. Harper, Scottish Crop Research Institute, UK, G. Himmler, University of Vienna, Austria, R. Kerschbaumer, University of Agriculture and Forestry, R. Koenig, Institut fur Biochemieund Pflanzenvirologie, M.A. Mayo, Scottish Crop Research Institute, UK, M. Peisker, Institut fur Pflankzengenetik und Kulturpflanzenforschung Gatersleben, Germany, J. Phillips, QIAGEN GmbH, Germany, A. Schots, Department of Nematology, The Netherlands, P.Seddas-Dozolme, INRA Colmar, France, G. Smant, Department of Nematology, The Netherlands, L. Torrance, Scottish Crop Research Institute, UK, R.L. Toth, Scottish Crop Research Institute, UK, M. Van Montagu, University of Gent, Belgium, M.H.V. VanRegenmortel, IBMC, France, B. Walter, INRA Colmar, France, G. Zedar-Lutz, IBMC, France, A. Ziegler, Scottish Crop Research Institute, UK.show more