RNA Methodologies

RNA Methodologies : A Laboratory Guide for Isolation and Characterization

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Description

The isolation of biologically competent and chemically stable RNA is central to molecular biology. As there is currently a need to unify components of RNA characterization, this text presents RNA isolation strategies and a collection of protocols in a coherent start-to-finish format. It is written for anyone capable of conducting basic research, and addresses basic skills involved in nucleic acid research as well as some of the more sophisticated protocols. A collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA, this book focuses on the study of RNA as a parameter of gene expression. "RNA Methodologies: A Laboratory Guide for Isolation and Characterization" emphasizes how these methodologies can be utilized in the study of transcriptional and post-transcriptional regulation of eukaryotic genes. The chapters work together to tell the RNA story, each including clear take-home lessons. The liberal incorporation of flow charts, tables and graphs facilitate learning and assist in the planning phases of a project.
The text studies cellular regulation using RNA as a parameter of gene expression, offers RNA isolation strategies and explains propert handling, storage and manipulation of RNA.
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Product details

  • Spiral bound | 331 pages
  • 157.48 x 226.06 x 12.7mm | 476.27g
  • Academic Press Inc
  • San Diego, United States
  • English
  • appendix, charts, tables, graphs
  • 0122497007
  • 9780122497001

Table of contents

RNA and the cellular biochemistry revisited - a rationale; transcription and the organization of eukaryotic gene sequences; messenger RNA; resilient ribonucleases; RNA isolation strategies; isolation of polyadenylated RNA; electrohoresis - principles and parameters; electrophoresis of RNA; the Northern blot; nucleic acid probe technology; practical nucleic acid hybridization; principles of detection; quantititation of specific mRNAs by the S1 nuclease assay; analysis of nuclear RNA; transcription rate assays; extraction of nuclear RNA for steady state analysis; an RNA paradigm.
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