RNA Methodologies

RNA Methodologies : A Laboratory Guide for Isolation and Characterization

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Description

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.

RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.
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Product details

  • Hardback | 688 pages
  • 162.6 x 223.5 x 38.1mm | 1,065.95g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 3rd edition
  • 0122496965
  • 9780122496967
  • 2,172,395

Table of contents

Chapter 1: RNA and the Cellular Biochemistry Revisited

Chapter 2: Transcription and the Organization of Eukaryotic Genes

Chapter 3: Messenger RNA

Chapter 4: Resilient Ribonucleases

Chapter 5: RNA Isolation Strategies

Chapter 6: The Truth About Tissues

Chapter 7: Isolation of Polyadenylated RNA

Chapter 8: Quality Control for RNA Preparations

Chapter 9: Dot Blot Analysis

Chapter 10: Electrophoresis of RNA

Chapter 11: Photodocumentation and Image Analysis

Chapter 12: Northern Analysis

Chapter 13: Nucleic Acid Probe Technology

Chapter 14: Practical Nucleic Acid Hybridization

Chapter 15: Principles of Detection

Chapter 16: Quantification of Specific mRNAs by Nuclease Protection

Chapter 17: Analysis of Nuclear RNA

Chapter 18: cDNA Synthesis

Chapter 19: RT-PCR

Chapter 20: Quantitative PCR Techniques

Chapter 21: Transcript Subtraction Methods

Chapter 22: mRNA Differential Display

Chapter 23: High-Throughput Analysis of Gene Expression

Chapter 24: RNA Interference: Targeted Gene Silencing

Chapter 25: Genomes, Transcriptomes, Proteomes, and Bioinformatics

Chapter 26: An RNA Paradigm

Epilogue: A Few Pearls of Wisdom

Appendix A: Maintaining Complete and Accurate Records

Appendix B: Useful Stock Solutions for the Molecular Biologist

Appendix C: Phenol Preparation

Appendix D: Disposal of Ethidium Bromide and SYBR Green Solutions

Appendix E: DNase I Removal of DNA from an RNA Sample

Appendix F: RNase Incubation to Remove RNA from a DNA Sample

Appendix G: Deionization of Formamide, Formaldehyde, and Glyoxal

Appendix H: Silanizing Centrifuge Tubes and Glassware

Appendix I: Centrifugation as a Mainstream Tool for the Molecular Biologist

Appendix J: Trypsinization Protocol for Anchorage-Dependent Cells

Appendix K: Isolation of High-Molecular-Weight DNA by Salting-Out

Appendix L: RNA Isolation from Plant Tissue

Appendix M: Electrophoresis: Principles, Parameters, and Safety

Appendix N: Polyacrylamide Gel Electrophoresis

Appendix O: Selected Suppliers of Equipment, Reagents, and Services

Appendix P: Useful SI Units

Appendix Q: Common Abbreviations

Appendix R: Trademark Citations

Glossary

Index
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Review Text

Praise for the third edition
"This third edition of RNA Methodologies is an updated version of a laboratory guide edited for the first time in 1993; it represents an exhaustive collection of tried, tested, and optimized protocols for isolation and chracterization of RNA. The third edition of RNA Methodologies is a well-documented guide for any researcher who has to manage RNA studies. Each chapter of this book provides a rationale to help in the decision-making process...The inclusion of troubleshooting hints and annotations based on personal experience are provided to improve the method(s). This laboratory guide offers protocols on traditional and new technologies, with a focus on how to use the expedient technique for achieving an experimental goal in the study of gene expression."
--Dr. Patrick Pagesy at INSERM for HEMOGLOBIN Praise for the third edition
"This third edition of RNA Methodologies is an updated version of a laboratory guide edited for the first time in 1993; it represents an exhaustive collection of tried, tested, and optimized protocols for isolation and chracterization of RNA. The third edition of RNA Methodologies is a well-documented guide for any researcher who has to manage RNA studies. Each chapter of this book provides a rationale to help in the decision-making process...The inclusion of troubleshooting hints and annotations based on personal experience are provided to improve the method(s). This laboratory guide offers protocols on traditional and new technologies, with a focus on how to use the expedient technique for achieving an experimental goal in the study of gene expression."
--Dr. Patrick Pagesy at INSERM for HEMOGLOBIN
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Review quote

"...an exhaustive collection of tried, tested, and optimized protocols for isolation and characterization of RNA."
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About Robert E. Farrell

Dr. Robert Farrell is a bench-current scientist who has 35 years of experience working with RNA in the study of transcriptional and posttranscriptional regulation of gene expression in a variety of model systems. He is also experienced in animal cell culture methods. Prior to joining the faculty at Penn State University, he operated a biotech education and service firm, winning the 1998 Small Business Contractor of the Year award from the U.S. Department of Agriculture. He is the recipient of campus- and college-wide awards for excellence in teaching, and has extensive experience running RNA and specialized biotechnology hands-on laboratory training programs all over the world. He often serves as a consultant within the pharmaceutical and biotech industries. Dr. Farrell received his Ph.D. and M.S. degrees from The Catholic University of America and his B.S. in Biology from Providence College. Dr. Farrell currently serves as the campus academic officer at Penn State York.
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