Quantitative Real-Time PCR

Quantitative Real-Time PCR : Methods and Protocols

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This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.
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Product details

  • Hardback | 235 pages
  • 178 x 254 x 22mm | 709g
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2020
  • 61 Tables, color; 55 Illustrations, color; 15 Illustrations, black and white; XIII, 235 p. 70 illus., 55 illus. in color.
  • 1493998323
  • 9781493998326
  • 1,164,645

Back cover copy

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.
show more

Table of contents

1. A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers



Alessandro Raso and Roberto Biassoni







2. Parameters for Successful PCR Primer Design



Stephen A. Bustin, Reinhold Mueller, and Tania Nolan







3. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing



Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, and Michael W. Pfaffl







4. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation



Valentin Vautrot and Isabelle Behm-Ansmant







5. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR



Priscilla Biswas, Eddi Di Marco, and Mauro S. Malnati







6. Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR)



Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constanca Britto, and Alice Gomes Fernandes Monteiro







7. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR)



Paula Maza-Marquez, Elisabet Aranda, Jesus Gonzalez-Lopez, and Belen Rodelas







8. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine



Nicola Luchi, Alberto Santini, Francesca Salvianti, and Pamela Pinzani







9. A General Protocol for Accurate Gene Expression Analysis in Plants



Ellen De Keyser, Laurence Desmet, Magali Losschaert, and Jan De Riek







10. Gene Expression Analysis in Bacteria by RT-qPCR



Danilo J.P.G. Rocha, Thiago L.P. Castro, Eric R.G.R. Aguiar, and Luis G.C. Pacheco







11. Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR



Francesca Salvianti, Filomena Costanza,Gemma Sonnati, and Pamela Pinzani







12. Molecular Monitoring of Chronic Myeloid Leukemia



Katherine Dominy, Katya Mokretar, Alistair G. Reid, and Jamshid S. Khorashad







13. Normalization in Human Gliomas Tissue



Ana Paula Santin Bertoni, Isabele Cristina Iser Marson, Rafael Paschoal de Campos, and Marcia Rosangela Wink







14. qPCR Applications for the Determination of the Biological Age



Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello, and Nicoletta Sacchi







15. QuantStudio (TM) 12K Flex OpenArray (R) System as a Tool for High-Throughput Genotyping and Gene Expression Analysis



Chiara Broccanello, Letizia Gerace, and Piergiorgio Stevanato







16. Digital PCR and the QuantStudio (TM) 3D Digital PCR System



Marion Laig, Christie Fekete, and Nivedita Majumdar
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