Pulsed Field Gel Electrophoresis

Pulsed Field Gel Electrophoresis : A Practical Guide

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Description

Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels.
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Product details

  • Hardback | 272 pages
  • 197.4 x 236.2 x 15.2mm | 527.51g
  • Academic Press Inc
  • San Diego, United States
  • English
  • photographs, appendices, references
  • 0121012905
  • 9780121012908

Table of contents

Introduction to Pulsed Field Gel Electrophoresis. PFGE Instrumentation. Preparation of DNA for Pulsed Field Analysis. Pouring Gels and Handling Samples. Restriction Enzyme Digestion of Large DNA Fragments in Agarose. Switch Intervals and Resolution in Pulsed Field Gels. Field Inversion Gel Electrophoresis (FIGE). Variables Which Affect Pulsed Field Gels. Southern Blot Analysis of Pulsed Field Gels. Special Applications. Trouble Shooting. Bibliography of Pulsed Field Gel References. Appendices. Chapter References.
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