Publications - Bureau of Government Laboratories Volume 11-21

Publications - Bureau of Government Laboratories Volume 11-21

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1904 edition. Excerpt: obtain pure cultures of uniebas. Pure cultm'es.--la11_' methods have been employed for this purpose, but few if any have ever been successful; and even in those 'ln keepin;_' stock cultures. transplants have been found successful with some illllelnls after six and one-half nmnth.-. while with others they have been found to die unless much more frequentl_v transferred. cases where the parasite was supposed to have been isolated, its satisfactory development never took place when transplants were made. ' Kartulis (1890) thought that, on a Inedium consisting of a straw decoction, he had obtained a pure culture of amebas from an abscess of the liver culturally free from bacteria. Ogata (1893) maintained that he had secured protozoa in pure culture. His method consisted essentially in taking advantage of the principle now quite generally known as negative geotropism, which is a movement peculiar to a number of the protozoa. Celli and Fiocca (1895) attempted to eliminate the bacteria by various methods, such as exposure of the mixed cultures to 55 and 60 C. for various lengths of time, filtration, placing disinfectants in the media, and inoculation into animals, thus endeavering to secure pure cultures. But when at length, after great pains, they succeeded in obtaining amebas free from bacteria, the former did not reproduce on transplants. Casagrandi and Barbagallo (1897) concluded that it was impossible to separate the amebas from the bacteria in the ordinary media. By the use of the Fucus crispus medium of Celli and Fiocca they secured cultures free from bacteria. They also succeeded in isolating amebas from a culture made with yeast. These writers revert to the original idea that the association of the protozoa and bacteria is...
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Product details

  • Paperback | 164 pages
  • 189 x 246 x 9mm | 304g
  • United States
  • English
  • black & white illustrations
  • 1236956494
  • 9781236956491