Proteomics in Practice : A Laboratory Manual of Proteome Analysis
This is a combined review, manual and reference for the successful analysis of proteins using the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries. The first section, written in a textbook style, introduces the entire technology, while the second section represents a comprehensive laboratory manual spanning the full range of methods from sample preparation to protein identification. Alternative methods and procedures are only suggested for those cases where the "default" procedure would fail to deliver adequate results. The third section is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further detail. Intended for all those wishing to go beyond the theoretical aspects of proteome analysis, this book is targeted at research groups within academia and industry, course instructors, research assistants and graduate students. Special feature: Updates of recipes as well as animations illustrating crucial methodological steps are available on the Web.
- Other digital | 350 pages
- 1 x 1 x 1mm
- 19 Jul 2003
- Wiley-VCH Verlag GmbH
- Weinheim, Germany
Table of contents
Preface Foreword Abbreviations, Symbols, Units Glossary of Terms PART I: PROTEOMICS TECHNOLOGY Introduction. Expression Proteomics. PART II: COURSE MANUAL. Equipment, Consumables, Reagents. Step 1: Sample Preparation. Step 2: Isoelectric Focusing. Step 3: SDS Polyacrylamide Gel Electrophoresis. Step 4: Straining of the Gels. Step 5: Scanning of Gels and Image Analysis. Step 6: Fluorescence Difference Gel Electrophoresis. Tep 7: Spot Excision. Step 8: Sample Destaining. Step 9: In--gel Digestion. Step 10: Microscale Desalting and Concentration of Sample. Step 11: Chemical Derivatisation of the Peptide Digest. Step 12: MS Analysis. Step 13: Calibration of the MALDI--ToF MS. Step 14: Preparing for a Database Search. Step 15: PMF Database Search Unsuccessful. Trouble Shooting. References. Index.
"This book says straight from the start that it will tell you how to do proteomics by 2D electrophoresis, followed by peptide mass fingerprinting, and then CAF MALDI sequence analysis" .................. ProteoMonitor