Proteins LabFax

Proteins LabFax

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Description

Proteins Labfax is a detailed and comprehensive compendium of information on protein chemistry, and the purification and physical characterization of proteins. The book provides data on all the procedures usedon a regular basis by researchers studying proteins, including chromatography, crystallization, size determination, computer-aided structure analysis, amino acid analysis, and the chemical modification of amino acid side chains. General data is also givenon buffers, salting out, criteria of purity, expression systems, and protein stability.The contents of Protein Labfax have been carefully chosen to complement the data presented in its companion volume, Enzymology Labfax.
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Product details

  • Hardback | 318 pages
  • 165 x 241 x 25.4mm | 860g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0125647107
  • 9780125647106

Table of contents

Purification:
Choice of Assay or Detection Method.
Preparation of Cell Extracts.
Buffers.
The Determination of Protein Concentration.
Salting Out: Ammonium Sulfate Precipitation.
Chromatographic Procedures.
Covalent Chromatography by Thiol-Disulfide Interchange using Solid-Phase Alkyl 2-Pyridyl Disulfide.
Criteria of Protein Purity.
Electrophoresis Methods.
Integral Membrane Proteins.
Expression Systems and Fusion Proteins.
Inclusion Bodies and Refolding.
Industrial Scale Purification of Proteins.
Peptide Synthesis.
Crystallization Procedures.
Physical Characterization:
Size Determination of Proteins: Hydrodynamic Methods and Mass Spectromic Methods.
FTIR and Raman Spectroscopies in the Study of Proteins and Other Biological Molecules.
Circular Dichroism.
NMR Structure Determination of Proteins.
EPR Spectroscopy of Proteins. Protein Stability.
Computer Analysis of Protein Structure.
Protein Chemistry:
Protein Chemistry Methods, Post-Translational Modification,Consensus Sequences.
Chemical Modification of Amino Acid Side-Chains.
Affinity-Based Covalent Modification.
Cross-Linking Reagents for Proteins.
Chapter References.
Subject Index.
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