Protein Purification Protocols

Protein Purification Protocols

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The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.show more

Product details

  • Hardback | 484 pages
  • 182.9 x 259.1 x 33mm | 1,088.63g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd ed. 2004
  • biography
  • 1588290670
  • 9781588290670

Review quote

From reviews of the first edition... "...very useful..." -Angewandte Chemie: International Edition "...valuable to keep around the laboratory..." -Biopharm "...recommended for protein chemists...There is something in this book for everyone...an excellent working book as well as an excellent reference." -JAOCS: Journal of the American Oil Chemists' Society "...an excellent lab companion..." -SIM News "...a worthwhile purchase for novice and established practitioners alike." -Microbiology Today "...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists." -Biomedical Chromatography "Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners."-SGM Quarterlyshow more

Back cover copy

In this new edition of the very successful Protein Purification Protocols (1996), Paul Cutler completely updates the existing protocols to reflect recent advances and adds an enormous new array of proteomic techniques for protein isolation and analysis. These cutting-edge techniques include not only two-dimensional gel electrophoresis for analysis and characterization, but also analytical chromatography for multidimensional separations of proteins and peptides, and mass spectrometry for isolating proteins. With the many recent advances in technology, simple spectrometric detection is no longer the only option for separating proteins, and the authors treat in full detail all the newer methods for these separations. Comprehensive and highly practical, Protein Purification Protocols, Second Edition, brings together all the key methodologies that both novice and experienced investigators need to carry out successful experimental work on proteins and their functions today.show more

Table of contents

General Strategies Shawn Doonan and Paul Cutler Preparation of Extracts From Animal Tissues J. Mark Skehel Protein Extraction From Plant Tissues Roger J. Fido, E. N. Clare Mills, Neil M. Rigby, and Peter R. Shewry Extraction of Recombinant Protein From Bacteria Anne F. McGettrick and D. Margaret Worrall Protein Extraction From Fungi Paul D. Bridge, Tetsuo Kokubun, and Monique S. J. Simmonds Subcellular Fractionation of Animal Tissues Norma M. Ryan Subcellular Fractionation of Plant Tissues: Isolation of Plastids and Mitochondria Alyson K. Tobin and Caroline G. Bowsher The Extraction of Enzymes From Plant Tissues Rich in Phenolic Compounds William S. Pierpoint Avoidance of Proteolysis in Extracts Robert J. Beynon and Simon Oliver (Revised by Paul Cutler) Concentration of Extracts Shawn Doonan Making and Changing Buffers Shawn Doonan Purification and Concentration by Ultrafiltration Paul Schratter (Revised by Paul Cutler) Bulk Purification by Fractional Precipitation Shawn Doonan Ion-Exchange Chromatography Chris Selkirk Hydrophobic Interaction Chromatography Paul A. O'Farrell Affinity Chromatography Paul Cutler Dye-Ligand Affinity Chromatography Anne F. McGettrick and D. Margaret Worrall Lectin Affinity Chromatography Iris West and Owen Goldring (Revised by Paul Cutler) Immunoaffinity Chromatography Paul Cutler Immobilized Metal-Ion Affinity Chromatography Tai-Tung Yip and T. William Hutchens (Revised by Paul Cutler) Chromatography on Hydroxyapatite Shawn Doonan Thiophilic Affinity Chromatography and Related Methods Paul Matejtschuk Affinity Precipitation Methods Jane A. Irwin and Keith F. Tipton Isoelectric Focusing Reiner Westermeier Chromatofocusing Timothy J. Mantle and Patricia Noone Size-Exclusion Chromatography Paul Cutler Fast Protein Liquid Chromatography David Sheehan and Siobhan O'Sullivan Reversed-Phase Chromatography of Proteins William A. Neville Extraction of Membrane Proteins Kay Ohlendieck Removal of Detergent From Protein Fractions Kay Ohlendieck Purification of Membrane Proteins Kay Ohlendieck Lyophilization of Proteins Ciaran O'Fagain Storage of Pure Proteins Ciaran O'Fagain Electroelution of Proteins From Polyacrylamide Gels Michael J. Dunn Electroblotting of Proteins From Polyacrylamide Gels Michael J. Dunn Two-Dimensional Polyacrylamide Gel Electrophoresis for Proteome Analyses Neil A. Jones Microscale Solution Isoelectrofocusing: A Sample Prefractionation Method for Comprehensive Proteome Analysis Xun Zuo and David W. Speicher Practical Column Chromatography Shawn Doonan Detection Methods Jacek Mozdzanowski and Sudhir Burman Peptide Proteomics Dean E. McNulty and J. Randall Slemmon Multidimensional Liquid Chromatography of Proteins Rod Watson and Tim Nadler Mass Spectrometry David J. Bell Purification of Therapeutic Proteins Julian Bonnerjea Purification Process Scale-Up Karl Prince and Martin Smith Indexshow more

Review Text

From reviews of the first edition... "...very useful..." -Angewandte Chemie: International Edition "...valuable to keep around the laboratory..." -Biopharm "...recommended for protein chemists....There is something in this book for everyone....an excellent working book as well as an excellent reference." -JAOCS: Journal of the American Oil Chemists' Society "...an excellent lab companion..." -SIM News "...a worthwhile purchase for novice and established practitioners alike." -Microbiology Today "...a detailed and comprehensive collection...up to date..invaluable to newcomers and of vast benefit to established researchers/scientists." -Biomedical Chromatography "Eight years on this is a welcome new edition which retains the same overall format of a strategies chapter followed by some 43 chapters, each dealing with individual techniques. Updating ranges from new introductions, to additional references and modified protocols. The final six chapters are new and include a useful comparison of detection methods and guides to proteomics and mass spectrometry. The larger format and hard binding make this edition appear less of a bench book, but the Editor has wisely retained the readily approachable format and enhanced its overall value as a practical manual, collating a wide range of protocols useful for both less and more experienced practitioners."-SGM Quarterlyshow more

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