Protein'Protein Interactions
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Protein'Protein Interactions : Methods and Applications

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As the mysteries stored in our DNA have been more completely revealed, scientists have begun to face the extraordinary challenge of unraveling the int- cate network of protein-protein interactions established by that DNA fra- work. It is increasingly clear that proteins continuously interact with one another in a highly regulated fashion to determine cell fate, such as proliferation, diff- entiation, or death. These protein-protein interactions enable and exert str- gent control over DNA replication, RNA transcription, protein translation, macromolecular assembly and degradation, and signal transduction; essentially all cellular functions involve protein-protein interactions. Thus, protein-p- tein interactions are fundamental for normal physiology in all organisms. Alt- ation of critical protein-protein interactions is thought to be involved in the development of many diseases, such as neurodegenerative disorders, cancers, and infectious diseases. Therefore, examination of when and how protein-p- tein interactions occur and how they are controlled is essential for understa- ing diverse biological processes as well as for elucidating the molecular basis of diseases and identifying potential targets for therapeutic interventions. Over the years, many innovative biochemical, biophysical, genetic, and computational approaches have been developed to detect and analyze p- tein-protein interactions. This multitude of techniques is mandated by the diversity of physical and chemical properties of proteins and the sensitivity of protein-protein interactions to cellular conditions.show more

Product details

  • Hardback | 532 pages
  • 157.48 x 231.14 x 30.48mm | 997.9g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2004 ed.
  • XVI, 532 p.
  • 1588291200
  • 9781588291202

Back cover copy

Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases, and identifying potential targets for therapeutic interventions. In Protein-Protein Interactions: Methods and Applications, leading experts describe in detail their highly successful biochemical, biophysical, genetic, and computational techniques for studying these interactions. Their readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics- and bioinformatics-based approaches, such as the Scansite program for computational analysis. Each time-tested protocol includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and highly practical, Protein-Protein Interactions: Methods and Applications offers both beginning and experienced investigators a full range of the powerful tools needed for deciphering how proteins interact to form biological networks, as well as for unraveling protein-protein interactions in disease in the search for novel therapeutic targets.show more

Review quote

From the reviews: "This book is thorough and packed with information...the volume is of high practical value and is fully recommended to all biochemists..." - Cell Biochemistry and Function"The book is well-balanced and easy to use...well worth the purchasing cost." - ChemBioChem "This ... volume comprehensively describes a variety of biophysical-, cell biology- and molecular biology-based approaches to the analysis of protein-protein interactions. ... This volume is recommended as a solid presentation on the fundamentals of protein interaction analysis, suitable for graduate biophysical chemistry courses and for those investigators new to the field who would like to get a solid grounding ... ." (Wayne F. Patton, Proteomics, Issue 6, 2006)show more

Table of contents

Part I. Overview Structural Basis of Protein-Protein Interactions Robert C. Liddington Quantitative Analysis of Protein-Protein Interactions Keith D. Wilkinson Part II. In Vitro Techniques Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry Adrian Velazquez-Campoy, Stephanie A. Leavitt, and Ernesto Freire Circular Dichroism Analysis for Protein-Protein Interactions Norma J. Greenfield Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy Guanghua Gao, Jason G. Williams, and Sharon L. Campbell Measuring Rhodopsin-G-Protein Interactions by Surface Plasmon Resonance John Northup Using Light Scattering to Determine the Stoichiometry of Protein Complexes Jeremy Mogridge Sedimentation Equilibrium Studies Ian A. Taylor, John F. Eccleston, and Katrin Rittinger Analysis of Protein-Protein Interactions by Simulation of Small-Zone Gel Filtration Chromatography Rosemarie Wilton, Elizabeth A. Myatt, and Fred J. Stevens Fluorescence Gel Retardation Assay to Detect Protein-Protein Interactions Sang-Hyun Park and Ronald T. Raines Fluorescence Polarization Assay to Quantify Protein-Protein Interactions Sang-Hyun Park and Ronald T. Raines Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot Randy A. Hall Glutathione-S-Transferase-Fusion-Based Assays for Studying Protein-Protein Interactions Haris G. Vikis and Kun-Liang Guan Affinity Capillary Electrophoresis Analyses of Protein-Protein Interactions in Target-Directed Drug Discovery William E. Pierceall, Lixin Zhang, and Dallas E. Hughes Mapping Protein-Ligand Interactions by Hydroxyl-Radical Protein Footprinting Nick Loizos Use of Phage Display and Polyvalency to Design Inhibitors of Protein-Protein Interactions Michael Mourez and R. John Collier Part III. Detecting Protein-Protein Interactions in Heterologous Systems A Bacterial Two-Hybrid System Based on Transcriptional Activation Simon L. Dove and Ann Hochschild Using the Yeast Two-Hybrid System to Identify Interacting Proteins John Miller and Igor Stagljar Analysis of Protein-Protein Interactions Utilizing Dual Bait Yeast Two-Hybrid System Ilya G. Serebriiskii and Elena Kotova The Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System Safia Thaminy, John Miller, and Igor Stagljar Reverse Two-Hybrid Techniques in the Yeast Saccharomyces cerevisiae Matthew A. Bennett, Jack F. Shern, and Richard A. Kahn Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions In Vivo Jae Woon Lee and Soo-Kyung Lee Co-Immunoprecipitation from Transfected Cells Shane C. Masters Part IV: Probing Protein-Protein Interactions in Living Cells Microscopic Analysis of Fluorescence Resonance Energy Transfer (FRET) Brian Herman, R. V. Krishnan, and Victoria E. Centonze Monitoring Molecular Interactions in Living Cells Using Flow Cytometric Analysis of Fluorescence Resonance Energy Transfer Francis Ka-Ming Chan Fluorescence Correlation Spectroscopy: A New Tool for Quantification of Molecular Interactions Keith M. Berland Confocal Microscopy for Intracellular Co-localization of Proteins Toshiyuki Miyashita Mapping Biochemical Networks with Protein-Fragment Complementation Assays Ingrid Remy and Stephen W. Michnick In Vivo Protein Cross-Linking Fabrice Agou, Fei Ye, and Michel Veron Part V. Proteomics-Based Approaches Computational Prediction of Protein-Protein Interactions John C. Obenauer and Michael B. Yaffe Affinity Methods forshow more

Review Text

From the reviews: "This book is thorough and packed with information...the volume is of high practical value and is fully recommended to all biochemists..." - Cell Biochemistry and Function "The book is well-balanced and easy to use...well worth the purchasing cost." - ChemBioChem "This ... volume comprehensively describes a variety of biophysical-, cell biology- and molecular biology-based approaches to the analysis of protein-protein interactions. ... This volume is recommended as a solid presentation on the fundamentals of protein interaction analysis, suitable for graduate biophysical chemistry courses and for those investigators new to the field who would like to get a solid grounding ... ." (Wayne F. Patton, Proteomics, Issue 6, 2006)show more

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