Protein Nanotechnology

Protein Nanotechnology : Protocols, Instrumentation, and Applications

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Leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near-field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens.show more

Product details

  • Hardback | 466 pages
  • 160 x 231.1 x 27.9mm | 884.52g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 195 black & white illustrations, biography
  • 1588293106
  • 9781588293107

Back cover copy

Rapidly emerging at the intersection of nanotechnology, materials science, and molecular biology, the field of nanobiotechnology promises to elucidate many life processes at the molecular-level previously invisible to human inquiry, and thereby dramatically to transform diagnostics, therapy, and drug discovery in this postgenomic world. In Protein Nanotechnology: Protocols, Instrumentation, and Applications, leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near-field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Protein Nanotechnology: Protocols, Instrumentation, and Applications provides investigators a wide variety of readily reproducible techniques and methods for exploring both protein functions and structures at the nanoscale level and their powerful new biological and medical applications.show more

Table of contents

Protein Nanotechnology: The New Frontier in Biosciences Tuan Vo-Dinh Kinetics and Mechanisms of Protein Crystallization at the Molecular Level Peter G. Vekilov Nanostructured Systems for Biological Materials Esther H. Lan, Bruce Dunn, and Jeffrey I. Zink Nanomaterials of Drug Delivery Systems for Tissue Regeneration Yasuhiko Tabata Nanotechnology With S-Layer Proteins Bernhard Schuster, Erika Gyorvary, Dietmar Pum, and Uwe B. Sleytr Folding of b-Structured Fibrous Proteins and Self-Assembling Peptides Anna Mitraki and Mark J. van Raaij Application of NMR Methods to Identify Detection Reagents for Use in Development of Robust Nanosensors Monique Cosman, Viswanathan V. Krishnan, and Rod Balhorn Studying 3D Subdomains of Proteins at the Nanometer Scale Using Fluorescence Spectroscopy Pierre M. Viallet and Tuan Vo-Dinh Carbon Nanotubes and Nanowires for Biological Sensing Jun Li, Hou Tee Ng, and Hua Chen Carbon Nanotube Systems to Communicate With Enzymes J. Justin Gooding and Joe G. Shapter Molecularly Imprinted Polymers for Biomolecular Recognition Alexandra Molinelli, Markus Janotta, and Boris Mizaikoff Plasmonics-Based Nanostructures for Surface-Enhanced Raman Scattering Bioanalysis Tuan Vo-Dinh, Fei Yan, and David L. Stokes Bacterial Virus f29 DNA-Packaging Motor and Its Potential Applications in Gene Therapy and Nanotechnology Peixuan Guo Construction of Ordered Protein Arrays Jarrod Clark, Taras Shevchuk, Piotr M. Swiderski, Rajesh Dabur, Laura E. Crocitto, Yaroslav I. Buryanov, and Steven S. Smith Bioengineering and Characterization of DNA-Protein Assemblies Floating on Supported Membranes Wilfrid Boireau, Anthony C. Duncan, and Denis Pompon Nanosystems for Biosensing: Multianalyte Immunoassay on a Protein Chip EiichiTamiya, Zheng-liang Zhi, Yasutaka Morita, and Quamrul Hasan Optical Nanosensors for Detecting Proteins and Biomarkers in Individual Living Cells Tuan Vo-Dinh Nanoelectrodes Integrated in Atomic Force Microscopy Cantilevers for Imaging of In Situ Enzyme Activity Angelika Kueng, Christine Kranz, Alois Lugstein, Emmerich Bertagnolli, and Boris Mizaikoff Protein Amyloidose Misfolding: Mechanisms, Detection, and Pathological Implications Nigil Satish Jeyashekar, Ajit Sadana, and Tuan Vo-Dinh Near-Field Scanning Optical Microscopy for Bioanalysis at Nanometer Resolution Musundi B. Wabuyele, Mustafa Culha, Guy D. Griffin, Pierre M. Viallet, and Tuan Vo-Dinh Indexshow more