Prostate Cancer Methods and Protocols

Prostate Cancer Methods and Protocols

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Prostate cancer is the second leading cancer in men in Western society. A major concern, and an area of intensive research, involves understanding why certain prostate cancers remain localized or indolent, whereas others become aggressive and metastasize. The differences between these cancer types have profound implications for patients and physicians. Indolent d- ease, which grows very slowly, generally does not cause any problems to the patient, whereas aggressive disease requires immediate treatment, the earlier the better. At present, there are no markers that discriminate between these two entities, thus causing a dilemma for the management of patients who have recently been diagnosed. The aim of Prostate Cancer Methods and Protocols is to explore cutting-edge molecular methods that may have the potential to reveal markers of disease for use in more accurate diagnoses of prostate c- cer and, consequently, to lead to new treatment strategies. This book provides a comprehensive collection of both in vitro and in vivo step-by-step protocols currently used by leaders in prostate cancer research, advice on approaches that can be used in the study of prostate cancer, as well as reviews covering areas less amenable to laboratory research, such as environmental factors in prostate cancer, to provide the reader with an overview of the prostate cancer research field as it currently stands.
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Product details

  • Hardback | 403 pages
  • 157.5 x 231.1 x 27.9mm | 771.12g
  • Totowa, NJ, United States
  • English
  • 2003 ed.
  • XII, 403 p.
  • 0896039781
  • 9780896039780

Back cover copy

With the prostate as the second leading site of cancer among males in Western society, the need to know why certain prostate cancers remain localized while others become aggressive and metastasize has become critical for the care of patients. In Prostate Cancer Methods and Protocols, leading investigators present basic and advanced molecular methods that reveal those markers essential for more accurate diagnoses of specific diseases, and in developing new treatment strategies. The techniques range from in vitro methods to in vivo models of prostate cancer. New methods for the accurate diagnosis of prostate cancer are also offered, including histological assessment, studies of cells in semen, and methylation analysis of the GSTpi, a potential prostate cancer-specific marker. Additional approaches, including proteome and microarray analyses, address such issues as assessing changes in the androgen receptor and studying the enzymes secreted by prostate cancer cells. New strategies for the treatment of refractory disease cover the role of flavonoids, targeted alpha therapy, methods for implementing immune therapy, and prostate-specific gene therapy. Each method is described in step-by-step detail to ensure successful results.
Comprehensive and cutting-edge, Prostate Cancer Methods and Protocols provides access to the many powerful tools needed by scientists engaged in prostate cancer research today, as well as up-to-date reviews on several key aspects of the disease.
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Table of contents

Epidemiological Investigation of Prostate Cancer
Graham G. Giles

Human Prostate Cancer Cell Lines
Pamela J. Russell and Elizabeth A. Kingsley

Growth of Prostatic Epithelial and Stromal Cells In Vitro
Donna M. Peehl

Prostate Epithelial Stem Cell Isolation and Culture
David L. Hudson and John R. W. Masters

Generation of Immortal Human Prostate Cell Lines for the Study of Prostate Cancer
Johng S. Rhim

Spheroids of Prostate Tumor Cell Lines
George Sgouros, Wei-Hong Yang, and Richard Enmon

Animal Models of Prostate Cancer
Pamela J. Russell and Dale J. Voeks

Transgenic Mouse Models for Prostate Cancer: Identification of an Androgen-Dependent Promoter and Creation and Characterization of the Long Probasin Promoter-Large T Antigen (LPB-Tag) Model
Susan Kasper, William Tu, Richard L. Roberts, and Scott B. Shappell

In Vivo Models of Human Prostate Cancer Bone Metastasis
Julie M. Brown

Effects of Fixation on Tissues
Elin Mortensen and Julie M. Brown

Background, Methods, and Protocols for the Histopathological Diagnosis of Prostate Carcinoma
Warick Delprado

Realizing the Potential of Ejaculate/Seminal Fluid in Detecting and Predicting Natural History
R. A. Gardiner, Michelle Burger, Judith A. Clements, and Martin F. Lavin

Bisulfite Methylation Analysis of Tumor Suppressor Genes in Prostate Cancer from Fresh and Archival Tissue Samples
Susan J. Clark, Douglas S. Millar, and Peter Molloy

Production and Characterization of Antipeptide Kallikrein 4 Antibodies: Use of Computer Modeling to Design Peptides Specific to Kallikrein 4
Tracey J. Harvey, Ying Dong, Loan Bui, Russell Jarrott, Terry Walsh, and Judith A. Clements

The Androgen Receptor CAG Repeat and Prostate Cancer Risk
Peter E. Clark, Ryan A. Irvine, and Gerhard A. Coetzee

Studies on Androgen Receptor Mutations and Amplification in Human Prostate Cancer
Zoran Culig, Alfred Hobisch, Martin Erdel, Georg Bartsch, and Helmut Klocker

Proteomics in the Analysis of Prostate Cancer
Soren Naaby-Hansen, Kohji Nagano, Piers Gaffney, John R. W. Masters, and Rainer Cramer

Application of Gene Microarrays in the Study of Prostate Cancer
Colleen C. Nelson, Douglas Hoffart, Martin E. Gleave, and Paul S. Rennie

Enhancer Trap Method Using a Green Fluorescent Protein Reporter Plasmid for Cloning Tissue-Specific Enhancers Active in Prostate Cells
Fujiko Watt and Peter Molloy

Targeted Alpha Therapy of Prostate Cancer
Barry J. Allen, Yong Li, Syed M. A. Rizvi, and Pamela J. Russell

Phenotypic and Functional Differences of Dendritic Cells Generated Under Different In Vitro Conditions
Stephanie E. B. McArdle, Selman A. Ali, Geng Li, Shahid Mian, and Robert C. Rees

Flavonoid Compounds in the Prevention and Treatment of Prostate Cancer
Graham E. Kelly and Alan J. Husband

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Review quote

"I know of no other book similar to this important compendium of methods for the study of prostate cancer. It is organized and edited skillfully and will be useful for any scientist-physician interested in the study of prostate cancer." -Doody's Health Sciences Book Review Journal

"...recommended for purchase by large health science or cancer research libraries. Or any library, which servies researchers and scientists, working on prostate cancer." - E-Streams
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