Practical Skills in Biomolecular Sciences
"Practical Skills in Biomolecular Science," is an indispensable book for undergraduate students in the life sciences. The book provides useful support at all stages of a degree course and underpins any practical course in biochemistry, biomedical science, genetics, immunology and microbiology. It is also a valuable resource for teachers of biology in colleges adn secondary schools. Laboratory and field studies are essential components of undergraduate training in biomolecular science. Pratcical work must be fully understood and effectively presented, but many students under-perform because they lack basic laboratory skills. This book, now in its second edition, continues to provide students with easy-to-use guidance for laboratory and field studies, but in addition it now covers broader transferable skills. As a result the new edition provides guidace and support over the entire range of a typical undergraduate course in biochemistry and biomedical science.
- Paperback | 552 pages
- 218 x 272 x 30mm | 1,360.79g
- 01 Jul 2003
- Pearson Education (US)
- Prentice Hall
- Upper Saddle River, United States
- 2nd edition
- 275 line drawings, index
Table of contents
Preface. For the Student. Acknowledgements. SECTION 1 STUDY AND EXAMINATION SKILLS. 1. The importance of transferable skills. 2. Managing your time. 3. Working with others. 4. Taking notes. 5. Revising. 6. Assessments and exams. 7. Preparing your CV. SECTION 2 INFORMATION TECHNOLOGY AND LIBRARY RESOURCES. 8. Finding and citing published information. 9. Evaluating information. 10. The Internet and World Wide Web. 11. Bioinformatics. 12.Using spreadsheets. 13.Word processors, databases and other packages. SECTION 3 COMMUNICATING INFORMATION. 14.Organizing a poster display. 15.Giving a spoken presentation. 16.General aspects of scientific writing. 17.Writing essays. 18.Reporting practical and project work. 19.Writing literature surveys and reviews. SECTION 4 FUNDAMENTAL LABORATORY TECHNIQUES. 20. Basic principles. 21. Health and safety. 22. Working with liquids. 23. Basic laboratory procedures. 24. Principles of soulution chemistry. 25. pH and buffer solutions. 26. Introduction to microscopy. 27. Setting up and using a light microscope. SECTION 5 THE INVESTIGATIVE APPROACH. 28. Making and recording measurements. 29. SI units and their use. 30. Scientific method and design of experiments. 31. Project work. SECTION 6 HANDLING CELLS AND TISSUE. 32. Sterile technique and microbial culture. 33. Isolating and indentifying microbes. 34. Working with animal and plant tissues and cells. 35. Culture systems and growth measurement. 36. Homogenization and fractionation of cells and tissues. SECTION 7 ANALYTICAL TECHNIQUES. 37. Calibration and its application to quantitative analysis. 38. Immunological methods. 39. Radioactive isotopes and their uses. 40. Light measurement. 41. Basic spectroscopy. 42. Advanced spectroscopy and spectrometry. 43. Centrifugation. 44. Chromatrography - separation methods. 45. Chromatrography - detection and analysis. 46. Principles and practice of electrophoresis. 47. Advanced electrophoretic techniques. 48. Electroanalytical techniques. SECTION 8 ASSAYING BIOMOLECULES AND STUDYING METABOLISM. 49. Analysis of biomolecules: fundamental principles. 50. Assaying amino acids and proteins. 51. Assaying lipids. 52. Assaying carbohydrates. 53. Assaying nucleic acids and nucleotides. 54. Protein purification. 55. Enzyme studies. 56. Membrane transport processes. 57. Photosynthesis and respiration. SECTION 9 GENETICS. 58. Mendelian genetics. 59. Bacterial and phage genetics. 60. Molecular genetics I - fundamental principles. 61. Molecular genetics II - PCR and related applications. 62. Molecular genetics III - genetic engineering techniques. SECTION 10 ANALYSIS AND PRESENTATION OF DATA. 63. Using graphs. 64. Presenting data in tables. 65. Hints for solving numerical problems. 66. Descriptive statistics. 67. Choosing and using statistical tests. Index.