Practical Flow Cytometry in Haematology
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Practical Flow Cytometry in Haematology : 100 Worked Examples

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The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis. This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis.
The reader will review May Grunwald Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference.
Practical Flow Cytometry in Haematology: 100 Worked Examples: * Provides a practical, example-based resource for flow cytometry * Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care * Includes both malignant and benign conditions * Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207) Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.
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Product details

  • Hardback | 414 pages
  • 192 x 252 x 24mm | 1,030g
  • New York, United States
  • English
  • 1. Auflage
  • 1118747038
  • 9781118747032
  • 64,251

Back cover copy

The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis.

This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors' institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis.

The reader will review May-Grünwald-Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference.

Practical Flow Cytometry in Haematology: 100 Worked Examples:

- Provides a practical, example-based resource for flow cytometry
- Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care
- Includes both malignant and benign conditions
- Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207)

Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.
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Table of contents

Preface, vii Acknowledgement, ix List of Abbreviations, xi Technical Notes, xv Laboratory Values, xix Case 1 1 Case 2 6 Case 3 11 Case 4 15 Case 5 18 Case 6 21 Case 7 24 Case 8 27 Case 9 31 Case 10 35 Case 11 39 Case 12 43 Case 13 46 Case 14 50 Case 15 54 Case 16 59 Case 17 62 Case 18 65 Case 19 68 Case 20 70 Case 21 74 Case 22 77 Case 23 80 Case 24 82 Case 25 87 Case 26 90 Case 27 93 Case 28 95 Case 29 100 Case 30 104 Case 31 106 Case 32 110 Case 33 114 Case 34 117 Case 35 122 Case 36 126 Case 37 129 Case 38 132 Case 39 136 Case 40 140 Case 41 143 Case 42 146 Case 43 151 Case 44 154 Case 45 159 Case 46 163 Case 47 166 Case 48 168 Case 49 172 Case 50 177 Case 51 180 Case 52 183 Case 53 186 Case 54 189 Case 55 193 Case 56 196 Case 57 201 Case 58 206 Case 59 210 Case 60 213 Case 61 216 Case 62 218 Case 63 224 Case 64 227 Case 65 232 Case 66 236 Case 67 240 Case 68 244 Case 69 249 Case 70 253 Case 71 256 Case 72 260 Case 73 266 Case 74 269 Case 75 274 Case 76 276 Case 77 281 Case 78 284 Case 79 289 Case 80 292 Case 81 297 Case 82 300 Case 83 306 Case 84 310 Case 85 315 Case 86 319 Case 87 321 Case 88 325 Case 89 327 Case 90 330 Case 91 334 Case 92 338 Case 93 342 Case 94 347 Case 95 351 Case 96 355 Case 97 359 Case 98 365 Case 99 370 Case 100 375 Antibodies Used in Immunohistochemistry Studies, 381 Flow Cytometry Antibodies, 386 Molecular Terminology, 389 Classification of Cases According to Diagnosis, 390 Index, 391
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About Mike Leach

Mike Leach FRCP, FRCPath Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK Mark Drummond PhD, FRCPath Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK Allyson Doig MSc, FIBMS Haemato-Oncology Laboratory Manager, Haematology Laboratories Gartnavel General Hospital, Glasgow, UK Pam McKay FRCP FRCPath Consultant Haematologist, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK Bob Jackson MB MRCPI FRCPath Haematopathology Laboratories, Southern General Hospital, Glasgow, UK Barbara J. Bain MB BS, FRACP, FRCPath, Professor of Diagnostic Haematology, St Mary s Hospital Campus of Imperial College Faculty of Medicine, London and Honorary Consultant Haematologist, St Mary s Hospital, London, UK
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