Plant Tissue Culture Manual

Plant Tissue Culture Manual : Supplement 6

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This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering. Both applied and more fundamental uses of cell, tissue and organ culture are covered, and the ring-binder design allows the manual to be updated regularly with supplementary chapters. Each chapter, discussing a single technique or protocol, is written by an authority in the field and provides background information, references to the relevant literature and a guide to troubleshooting in addition to a thorough methodology. This Sixth Supplement to the Plant Tissue Culture Manual expands the existing sections B: Tissue Culture & Transformation of Crop Species, C: Propagation & Conservation of Germplasm, F: Mutant Selection, and H: Tissue Culture Techniques for Fundamental Studies.
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Product details

  • Book | 212 pages
  • 157.5 x 236.2 x 10.2mm | 385.56g
  • Dordrecht, Netherlands
  • English
  • 1996 ed.
  • 6 Illustrations, black and white; 212 p. 6 illus.
  • 079233874X
  • 9780792338741

Table of contents

Section A: Basic Techniques - Cell & Tissue Culture of Model Species.- 1. Media preparation.- 2. The initiation and maintenance of callus cultures of carrot & tobacco.- 3. The initiation and maintenance of plant cell suspension cultures.- 4. Shoot cultures and root cultures of tobacco.- 5. Somatic embryogenesis in orchardgrass.- 6. Arabidopsis regeneration and transformation (leaf & cotyledon expiant system).- 7. Arabidopsis protoplast transformation and regeneration.- 8. Arabidopsis regeneration and transformation (root expiant system).- 9. Somatic embryogenesis in carrot.- 10. Low density cultures: microdroplets and single-cell nurse cultures.- 11. Tobacco protoplast isolation, culture and regeneration.- Section B: Tissue Culture & Transformation of Crop Species.- 1. Embryogenic callus, cell suspension and protoplast cultures of cereals.- 2. Transformation and regeneration of rice protoplasts.- 3. Transformation and regeneration of orchardgrass protoplasts.- 4. Transformation and regeneration of oilseed rape protoplasts.- 5. Regeneration and transformation of potato by Agrobacterium tumefaciens.- 6. Transformation of tomato with Agrobacterium tumefaciens.- 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens.- 8. Regeneration and transformation of apple (Malus pumila Mill.).- 9. Transformation and regeneration of maize protoplasts.- 10. Regeneration and transformation of barley protoplasts.- 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation.- 12. Production of fertile transgenic wheat by microprojectile bombardment.- 13. Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment.- Section C: Propagation & Conservation of Germplasm.- 1. Clonal propagation of orchids.- 2. Clonal propagation of palms.- 3. Clonal propagation of conifers.- 4. Cytological techniques.- 5. Restriction fragment analysis of somaclones.- 6. Virus elimination and testing.- 7. Clonal propagation of Citrus.- 8. Clonal propagation of eucalypts.- 9. Cryopreservation of plant tissue cultures: the example of embryogenie tissue cultures from conifers.- Section D: Direct Gene Transfer & Protoplast Fusion.- 1. Gene transfer by particle bombardment.- 2. Transformation of pollen by particle bombardment.- 3. Electrical fusion of protoplasts.- 4. Cybrid production and selection.- 5. Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids.- 6. RFLP analysis of organellar genomes in somatic hybrids.- 7. Isolation and uptake of plant nucle.- 8. In situ hybridization to plant metaphase chromosomes: Radioactive and non-radioactive detection of repetitive and low copy number genes.- 9. Chemical fusion of protoplasts.- Section E: Reproductive Tissues.- 1. In vitro fertilisation of maize.- 2. Endosperm culture.- 3. Endosperm culture.- 4. Hybrid embryo rescue.- 5. In vitro culture of Brassica juncea zygotic proembryos.- Section F: Mutant Selection.- 1. Use of chemical and physical mutagens in vitro.- 2. In vitro culture, mutant selection, genetic analysis and transformation of Physcomitrella patens.- Section G: Secondary Metabolites.- 1. Tropane alkaloid biosynthesis in vitro.- 2. Anthocyanin biosynthesis in vitro.- 3. Biosynthesis of monoterpene indole alkaloids in vitro.- Section H: Tissue Culture Techniques for Fundamental Studies.- 1. Establishment of photoautotrophic cell cultures.- 2. Zinnia mesophyll culture system to study xylogenesis.- 3. Cell cycle studies: induction of synchrony in suspension cultures of Catharanthus roseus cells H. Kodama.- 4. Thin Cell Layer (TCL) method to programme morphogenetic patterns.- 5. In vitro infection of Arabidopsis with nematodes.
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