Plant Molecular Biology Manual: Supplement III to 2r.e
This updated manual contains both fundamental and recently-described techniques in the area of plant molecular biology. Designed for use in the research laboratory, it presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and "in vitro" transcription systems, "in situ" hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This edition contains more than 40 new chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and "in vitro" transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition. The second edition includes Supplement III. Further supplements will be published regularly.
- Book | 142 pages
- 06 Apr 1998
- Kluwer Academic Publishers
- United States
- 2nd edition
- 12 black & white illustrations, 1 colour illustrations, biography
Table of contents
Supplement I: F4. Polymerase chain reaction: gene detection, inverse PCR, and genetic engineering; J. Slightom, et al. G3. Light microscopic applications of immunocytochemistry; K. Vandenbosch, et al. K3. T-DNA tagging in Arabidopsis thaliana: Cloning by gene disruption; B. Schulz, et al. L1. High level gene expression in plants using RNA viruses as transient expression vectors; C.M. Kearney, et al. L2. Geminivirus mediated gene transfer; D. Bisaro, G. Sunter. L3. Caulimovirus mediated gene transfer; I. Jupin, B. Gronenborn. Supplement II: RNA differential display; M.J. Laskowski. Expression analysis of multigene Families by RFLP-coupled domain-directed differential display (RC4D); A. Fischer, et al. Recombinant aequorin methods for measurement of intracellular calcium in plants; H. Knight, et al. GUS activity staining - A powerful tool in plant molecular biology; Jeong Hee Lee, F. Schoffl. In-Cell RT-PCR in a single, detached plant cell; Ho-Hyung Woo. Supplement III: A3. Liposome-mediated transfer of YAC-DNA to tobacco cells; M. Van Wordragen, et al. B7. Agribacterium germ-line transformation: Transformation of Arabidopsis without tissue culture; A.F. Bent, S. Clough. D6. RNA stability of chloroplasts; J. Nickelsen. G5. Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH); P. Fransz, et al. L4. Tobamovirus transient expression vectors: Tools for plant biology and high-level expression of foreign proteins in plants; G.P. pogue, et al. M1. Identification and characterization of protein interactions using the yeast 2-hybrid system; S.E. Kohalmi, et al.