Plant Microtechnique and Microscopy

Plant Microtechnique and Microscopy

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Plant Microtechnique and Microscopy surpases all previously written manuals by providing an in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, flourescence histochemistry, and in situ hybridization, are documented thoroughly. Preceding Chapter One are ten single-page "Quick Start" protocols. Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice (chemical toxicites, common calculations, and buffer tables) are provided to make this volume a valuable addition to every biological laboratory.
Plant Microtechnique and Microscopy contains a definitive chapter on microscopy and, with diagrams and text, describes the optical principles of techniques such as phase contrast, DIC, confocal and deconvolution wide-field microscopy. Also included is an extensive appendix on optics and its application to the microscope. The book's bibliography of over 650 references is a substantial resource for any student of histology, histological technique, or microscopy.
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Product details

  • Paperback | 336 pages
  • 215 x 280 x 20mm | 748.44g
  • New York, United States
  • English
  • numerous figures and tables
  • 0195089561
  • 9780195089561
  • 741,452

Table of contents

1. Quick Start ; Paraffin Embedding ; Paraffin Embedding - Microwave method ; Glycol methacrylate embedding ; Butyl/methyl methacrylate embedding ; Steedman's wax embedding ; Chromosome squashes ; Hematoxylin staining - microwave technique ; Kohler illumination ; 2. Microscopy ; History of the compound microscope ; Principles of specimen illumination ; 3. Chemical Fixation of Tissues ; The quality of fixation ; The mechanics of fixation ; Coagulating Fixatives ; Crosslinking Agents ; Other fixation methods ; Recommendations for fixing plant tissues ; 4. Tissue Dehydration ; General protocol ; Dehydration using a graded dehydration series ; Dehydration using chemical agents ; 5. Infiltrating Tissues ; Paraffin infiltrating ; Paraffin and PEG Embedding ; Polyethylene glycol and derivatives ; Plastic infiltrating ; 6. Sectioning and Mounting ; Mounting blocks to microtome stubs ; The quality of sectioning ; The microtome knife ; Setting up the microtome ; Sectioning Methods ; Mounting sections to glass slides ; 7. Staining ; Dye Chemistry ; Staining equipment ; General histological staining ; Other microtechniques stains and protocols ; Mounting the coverslip ; Coverslip mounting media ; 8. Alternate Methods of Microtomy ; Free-hand Sections ; Vibratome ; Cryostat ; Sliding microtome ; 9. Special Methods ; Clearing tissues with NaOH and chloral hydrate ; Clearing tissues without removing cytoplasmic components ; Macerating woody tissues ; Macerating non-woody tissues ; Bleaching tissues ; Preserving color in whole mounted specimens ; 10. Problem Solving ; Tissue collection ; Fixation ; Tannins ; Hard materials ; Sliding microtome ; Clearing ; Dehydration, Infiltration, and Embedding ; Microtomy ; Hand sections ; Staining ; 11. Histochemistry and Cytochemistry ; The relationship of the probe to the target ; Cytochemical localization of cell components ; Cytochemical localization of enzymes ; Cytochemical Fluorescence Microscopy ; 12. Localization of Molecular Targets ; Immunolocalization ; Tissue Printing ; in situ hybridization ; APPENDIX I: TOXICS ; APPENDIX II: BUFFERS ; APPENDIX III: COMMON CALCULATIONS ; APPENDIX IV: MERCURY ARC LAMP ; APPENDIX V: MANUFACTURERS AND VENDORS ; APPENDIX VI: OPTICS ; Properties of Light ; General lens optics ; The microscope objective ; The microscope eyepiece ; The condensor ; Fluorescence microscopy ; Microscopy light sources ; BIBLIOGRAPHY ; INDEX
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Review quote

"I am grateful to Steve Ruzin for assembling this incredibly useful collection of information in such a convenient and handy form." Plant Systematics & Evolution "an archive of expertise in the histochemistry field" Cell Biology International
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