Plant Cell Culture Protocols

Plant Cell Culture Protocols

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A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.show more

Product details

  • Hardback | 394 pages
  • 157.5 x 231.1 x 27.9mm | 771.12g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • Revised
  • 2nd Revised edition
  • 3 Illustrations, color; 74 Illustrations, black and white; XVI, 394 p. 77 illus., 3 illus. in color.
  • 1588295478
  • 9781588295477

Review quote

From Reviews of the First Edition... "...an excellent addition to the library of researchers who wish to extend their collection of basic cell culture techniques" -Quarterly Review of Biology "...represents a valuable resource of information on in vitro culture methods in plants, which will find extensive application on the laboratory bench." -Plant Science "...thoroughly recommend[ed]..." -Plant Growth Regulation "...undoubtedly represents one of the highest quality manuals on this topic published within the last ten years." -Biologia Plantarumshow more

Back cover copy

Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites. Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.show more

Table of contents

Part I. Introduction An Introduction to Plant Cell Culture: Back to the Future Victor M. Loyola-Vargas and Felipe Vazquez-Flota History of Plant Tissue Culture Trevor A. Thorpe Part II. Cell Culture and Plant Regeneration: The Fundamentals Pathogen and Biological Contamination Management: The Road Ahead Alan C. Cassells and Barbara M. Doyle Growth Measurements: Estimation of Cell Division and Cell Expansion Gregorio Godoy-Hernandez and Felipe A. Vazquez-Flota Callus and Suspension Culture Induction, Maintenance, and Characterization Alejandrina Robledo-Paz, Maria Nelida, Vazquez-Sanchez, Rosa Maria Adame-Alvarez, and Alba Estela Jofre-Garfias Measurement of Cell Viability in In Vitro Cultures Lizbeth A. Castro-Concha, Rosa Maria Escobedo, and Maria de Lourdes Miranda-Ham Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification Qiaochun Wang and Avihai Perl Somatic Embryogenesis in Picea Suspension Cultures Claudio Stasolla Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes Krit Raemakers, Isolde Pereira, Herma Koehorst van Putten, and Richard Visser Direct Somatic Embryogenesis in Coffea canephora Francisco Quiroz-Figueroa, Miriam Monforte-Gonzalez, Rosa M. Galaz-Avalos, and Victor M. Loyola-Vargas Part III. Plant Propagation In Vitro A New Temporary Immersion Bioreactor System for Micropropagation Manuel L. Robert, Jose Luis Herrera-Herrera, Gaston Herrera-Herrera, Miguel Angel Herrera-Alamillo, and Pedro Fuentes-Carrillo Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro Gabriela Fuentes, Carlos Talavera, Yves Desjardins, and Jorge M. Santamaria Use of Statistics in Plant Biotechnology Michael E. Compton AnEfficient Method for the Micropropagation of Agave Species Manuel L. Robert, Jose Luis Herrera-Herrera, Eduardo Castillo, Gabriel Ojeda, and Miguel Angel Herrera-Alamillo Micropropagation of Endangered Plant Species Zhihua Liao, Min Chen, Xiaofen Sun, and Kexuan Tang Clonal Propagation of Softwoods Trevor A. Thorpe, Indra S. Harry, and Edward C. Yeung Part IV. Applications for Plant Protoplasts Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora Michael R. Davey, Paul Anthony, J. Brian Power, and Kenneth C. Lowe Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts Zeng-guang Pan, Chun-zhao Liu, Susan J. Murch, and Praveen K. Saxena Production of Cybrids in Brassicaceae Species Maksym Vasylenko, Olga Ovcharenko, Yuri Gleba, and Nikolay Kuchuk Guard Cell Protoplasts: Isolation, Culture, and Regeneration of Plants Gary Tallman Production of Interspecific Hybrid Plants in Primula Juntaro Kato and Masahiro Mii Part V. Protocols for Genomic Manipulation Agrobacterium-Mediated Transformation of Petunia Leaf Discs Ingrid M. van der Meer Transformation of Wheat Via Particle Bombardment Indra K. Vasil and Vilma Vasil Chloroplast Transformation Xiao-Mei Lu, Wei-Bo Yin, and Zan-Min Hu The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers Cesar De los Santos-Briones and Teresa Hernandez-Sotomayor Transformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System Zuo-yu Zhao and Jerry Ranch Part VI. Accumulation of Metabolites in Plant Cells Capsaicin Accumulation in Capsicum spp. Suspension Cultures Neftali Ochoa-Alejo Isolation and Purification of Ribosome-Inactivating Proteins Sang-Wook Park,show more

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