Placenta and Trophoblast

Placenta and Trophoblast : Methods and Protocols, Volume II

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The aim of the two-volume set of Placenta and Trophoblast: Methods and Protocols is to offer contemporary approaches for studying the biology of the placenta. The chapters contained herein also address critical features of the female organ within which the embryo is housed, the uterus, and some aspects of the embryo-fetus itself, particularly those of common experimental animal models. In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty. In both volumes, sources of supplies are given and illustrations highlight particular techniques as well as expected outcomes. A key aspect of these volumes is that the contributors are at the forefronts of their disciplines, thus ensuring the accuracy and usefulness of the chapters. Placenta research has progressed rapidly over the past several decades by taking advantage of the technical advances made in other fields. For example, the reader will note that many techniques, such as reverse transcriptase po- merase chain reaction, northern and western blotting, microarray analyses and in situ hybridization experiments, are routinely used for dissecting a wide range of experimental questions. Protein analysis and functional experiments on tissues and cells that comprise the maternal-fetal interface benefit from studies in en- crinology, immunology, and developmental biology. These volumes also present new ideas on investigating gene imprinting and gene transfer via viral more

Product details

  • Hardback | 452 pages
  • 160 x 235 x 30mm | 898.13g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2006 ed.
  • XXI, 452 p.
  • 1588296083
  • 9781588296085

Review quote

"...will provide an excellent laboratory resource for years to come." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journal " important contribution to the literature, and I recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journalshow more

Back cover copy

Placental research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 2 provides readily reproducible protocols for studying trophoblast invasion, maternal immune cell responses to pregnancy, and the hormone-producing capabilities of the placenta. There are also methods to investigate the barrier function of the placenta, its role in regulating the transport of nutrients and wastes, and the adaptations of trophoblast cells and the placenta to disease. Highlights include techniques to analyze trophoblast cell interactions with the uterine vasculature and immune cells, and to the endocrine functions of the placenta. A companion first volume concentrates on methods for investigate embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of more

Table of contents

Part I. Introduction Placenta and Trophoblast: Methods and Protocols: Overview II Michael J. Soares and Joan S. Hunt Part II. Analysis of Trophoblast Invasion In Vivo Analysis of Trophoblast Cell Invasion in the Human Robert Pijnenborg, Elizabeth Ball, Judith N. Bulmer, Myriam Hanssens, Stephen C. Robson, and Lisbeth Vercruysse In Vitro Analysis of Trophoblast Invasion John D. Aplin An In Vitro Model of Trophoblast Invasion of Spiral Arteries Judith E. Cartwright and Mark Wareing Part III. Analysis of Uteroplacental Immune Cells and Their Functions In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells B. Anne Croy and Xuemei Xie Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface Thaddeus G. Golos, Gennadiy I. Bondarenko, Edith E. Breburda, Svetlana V. Dambaeva, Maureen Durning, and Igor I. Slukvin Methods for Isolation of Cells from the Human Fetal-Maternal Interface Anita Trundley, Lucy Gardner, Jacquie Northfield, Chiwen Chang, and Ashley Moffett In Vitro Models for Studying Human Uterine and Placental Macrophages Ramsey H. McIntire, Margaret G. Petroff, Teresa A. Phillips, and Joan S. Hunt Macrophage-Trophoblast Interactions Gil Mor, Shawn L. Straszewski-Chavez, and Vikki M. Abrahams Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon Daudi K. Langat, Asgerally T. Fazleabas, and Joan S. Hunt Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins Judith L. Pace, Pedro J. Morales, Teresa A. Phillips, and Joan S. Hunt Part IV. Analysis of Placental Function: Transport and Endocrinology In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport Timothy R. H. Regnault and William W. Hay, Jr. In Vitro Models for Studying Trophoblast Transcellular Transport Claudia J. Bode, Hong Jin, Erik Rytting, Peter S. Silverstein, Amber M. Young, and Kenneth L. Audus In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Plasma Membrane Vesicles Jocelyn D. Glazier and Colin P. Sibley In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Villous Fragments Susan L. Greenwood and Colin P. Sibley Methods for Investigating Placental Fatty Acid Transport Yan Xu, Thomas J. Cook, and Gregory T. Knipp Heterologous Expression Systems for Studying Placental Transporters Vadivel Ganapathy, You-Jun Fei, and Puttur D. Prasad Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures Noa Sher and Joseph Orly Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells Jonathan A. Green and R. Michael Roberts Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands Heiner Muller and Michael J. Soares Bacterial Expression of Prolactin Family Proteins Arieh Gertler Analysis of Placental Regulation of Hematopoiesis Beiyan Zhou and Daniel I. H. Linzer Methods for Studying Interferon Tau Stimulated Genes Fuller W. Bazer and Thomas E. Spencer Part V. Analysis of Placenta Adaptation to Disease Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia Joey P. Granger, B. Babbette D. LaMarca, Kathy Cockrell, Mona Sedeek, Charles Balzi, Derrick Chandler, and William Bennett In Vivo Rat Model of Preeclampsia S. Ananth Karumanchi and Isaac E. Stillman A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice Ana Claudiashow more