Placenta and Trophoblast
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Placenta and Trophoblast : Methods and Protocols, Volume I

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A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.show more

Product details

  • Hardback | 470 pages
  • 160 x 240 x 30mm | 948.02g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 2006.
  • XIX, 470 p.
  • 1588294048
  • 9781588294043

Review quote

"...provides step-by-step instructions for conducting research in mammalian embryology." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journal "...an important contribution to the literature...recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 93 - 4 Stars!-Doody's Health Science Book Review Journalshow more

Back cover copy

Placenta research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of the trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 1 provides readily reproducible protocols for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function. Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.show more

Table of contents

Part I. Introduction Placenta and Trophoblast: Methods and Protocols: Overview I Michael J. Soares and Joan S. Hunt Part II. Methods for Studying Embryo Implantation and Uterine Biology Methodologies to Study Implantation in Mice Kaushik Deb, Jeff Reese, and Bibhash C. Paria Blastocyst Culture D. Randall Armant Isolation of Hormone Responsive Uterine Stromal Cells: An In Vitro Model for Stromal Cell Proliferation and Differentiation Virginia Rider Rat Decidual Cell Cultures Yan Gu and Geula Gibori The Immortalization of Human Endometrial Cells Graciela Krikun, Gil Mor, and Charles Lockwood Sheep Uterine Gland Knockout (UGKO) Model Thomas E. Spencer and C. Allison Gray A Baboon Model for Inducing Endometriosis Asgerally T. Fazleabas A Baboon Model for Simulating Pregnancy Asgerally T. Fazleabas The Common Marmoset Monkey as a Model for Implantation and Early Pregnancy Research Almuth Einspanier, Kai Lieder, Ralf Einspanier, and Bettina Husen Part III. In Vitro Trophoblast and Placental Model Systems Mouse Trophoblast Stem Cells Jennifer Quinn, Tilo Kunath, and Janet Rossant Connexins and Trophoblast Cell Lineage Development Mark Kibschull and Elke Winterhager Rcho-1 Trophoblast Stem Cells: A Model System for Studying Trophoblast Cell Differentiation Namita Sahgal, Lindsey N. Canham, Brent Canham, and Michael J. Soares Bovine Trophoblast Cell Culture Systems: A Technique to Culture Bovine Trophoblast Cells Without Feeder Cells Kazuyoshi Hashizume, Arata Shimada, Haruo Nakano, and Toru Takahashi In Vitro Induction of Trophoblast from Human Embryonic Stem Cells Ren-He Xu Isolation and Culture of Term Human Trophoblast Cells Margaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, andJoan S. Hunt Production of Human Trophoblast Cell Lines Guy St J. Whitley Culture and Transfection of Human Choriocarcinoma Cells Michael W. Wolfe In Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the Chorion Karen M. Downs Part IV. Phenotypic Analysis of the Placenta Phenotypic Analysis of the Mouse Placenta David R. C. Natale, Maja Starovic, and James C. Cross Phenotypic Analysis of the Rat Placenta Rupasri Ain, Toshihiro Konno, Lindsey N. Canham, and Michael J. Soares Analysis of the Structure of the Ruminant Placenta: Methods of Fixation, Embedding, and Antibody Localization at Light and Electron Microscope Levels F. B. P. Wooding Characterization of the Bovine Placenta by Cytoskeleton, Integrin Receptors, and Extracellular Matrix Christiane D. Pfarrer Molecular Markers for Human Placental Investigation Berthold Huppertz Correlative Microscopy of Ultrathin Cryosections in Placental Research Toshihiro Takizawa and John M. Robinson Vascular Corrosion Casting of the Uteroplacental and Fetoplacental Vasculature in Mice Kathie J. Whiteley, Christiane D. Pfarrer, and S. Lee Adamson Analysis of Fetal and Maternal Microvasculature in Ruminant Placentomes by Corrosion Casting Rudolf Leiser and Christiane D. Pfarrer Part V. Molecular Analysis and Gene Transfer Techniques Microarray Analysis of Trophoblast Cells Vikram Budhraja and Yoel Sadovsky Gene Expression Microarray Data Analysis of Decidual and Placental Cell Differentiation Sue Kong, Bruce J. Aronow, and Stuart Handwerger Assays to Determine Allelic Usage of Gene Expression in the Placenta Paul B. Vrana Adenoviral-Mediated Gene Delivery to Trophoblast Cells Bing Jiang and Carole R. Mendelson Indexshow more