Placenta and Trophoblast

Placenta and Trophoblast : Methods and Protocols, Volume II

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The aim of the two-volume set of Placenta and Trophoblast: Methods and Protocols is to offer contemporary approaches for studying the biology of the placenta. The chapters contained herein also address critical features of the female organ within which the embryo is housed, the uterus, and some aspects of the embryo-fetus itself, particularly those of common experimental animal models. In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty. In both volumes, sources of supplies are given and illustrations highlight particular techniques as well as expected outcomes. A key aspect of these volumes is that the contributors are at the forefronts of their disciplines, thus ensuring the accuracy and usefulness of the chapters. Placenta research has progressed rapidly over the past several decades by taking advantage of the technical advances made in other fields. For example, the reader will note that many techniques, such as reverse transcriptase po- merase chain reaction, northern and western blotting, microarray analyses and in situ hybridization experiments, are routinely used for dissecting a wide range of experimental questions. Protein analysis and functional experiments on tissues and cells that comprise the maternal-fetal interface benefit from studies in en- crinology, immunology, and developmental biology. These volumes also present new ideas on investigating gene imprinting and gene transfer via viral vectors.
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Product details

  • Paperback | 452 pages
  • 154.94 x 234.95 x 27.43mm | 725.74g
  • Totowa, NJ, United States
  • English
  • 2006 ed.
  • XXI, 452 p.
  • 1627038639
  • 9781627038638

Back cover copy

Placental research has progressed rapidly over the past several decades by taking advantage of technical advances, such as microarray analysis, reverse transcriptase polymerase chain reaction, protein analysis, and in situ hybridization. In Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation, and function. Volume 2 provides readily reproducible protocols for studying trophoblast invasion, maternal immune cell responses to pregnancy, and the hormone-producing capabilities of the placenta. There are also methods to investigate the barrier function of the placenta, its role in regulating the transport of nutrients and wastes, and the adaptations of trophoblast cells and the placenta to disease. Highlights include techniques to analyze trophoblast cell interactions with the uterine vasculature and immune cells, and to the endocrine functions of the placenta. A companion first volume concentrates on methods for investigate embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta.
Comprehensive and state-of-the-art, Placenta and Trophoblast: Methods and Protocols, Volumes 1 & 2 provide researchers a firm foundation for successful cellular and molecular analysis of the placenta and the establishment of pregnancy.
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Table of contents

Part I. Introduction

Placenta and Trophoblast: Methods and Protocols: Overview II
Michael J. Soares and Joan S. Hunt

Part II. Analysis of Trophoblast Invasion

In Vivo Analysis of Trophoblast Cell Invasion in the Human
Robert Pijnenborg, Elizabeth Ball, Judith N. Bulmer, Myriam Hanssens, Stephen C. Robson, and Lisbeth Vercruysse

In Vitro Analysis of Trophoblast Invasion
John D. Aplin

An In Vitro Model of Trophoblast Invasion of Spiral Arteries
Judith E. Cartwright and Mark Wareing

Part III. Analysis of Uteroplacental Immune Cells and Their Functions

In Vivo Models for Studying Homing and Function of Murine Uterine Natural Killer Cells
B. Anne Croy and Xuemei Xie

Immune and Trophoblast Cells at the Rhesus Monkey Maternal-Fetal Interface
Thaddeus G. Golos, Gennadiy I. Bondarenko, Edith E. Breburda, Svetlana V. Dambaeva, Maureen Durning, and Igor I. Slukvin

Methods for Isolation of Cells from the Human Fetal-Maternal Interface
Anita Trundley, Lucy Gardner, Jacquie Northfield, Chiwen Chang, and Ashley Moffett

In Vitro Models for Studying Human Uterine and Placental Macrophages
Ramsey H. McIntire, Margaret G. Petroff, Teresa A. Phillips, and Joan S. Hunt

Macrophage-Trophoblast Interactions
Gil Mor, Shawn L. Straszewski-Chavez, and Vikki M. Abrahams

Methods for Evaluating Histocompatibility Antigen Gene Expression in the Baboon
Daudi K. Langat, Asgerally T. Fazleabas, and Joan S. Hunt

Analysis of the Soluble Isoforms of HLA-G: mRNAs and Proteins
Judith L. Pace, Pedro J. Morales, Teresa A. Phillips, and Joan S. Hunt

Part IV. Analysis of Placental Function: Transport and Endocrinology

In Vivo Techniques for Studying Fetoplacental Nutrient Uptake, Metabolism, and Transport
Timothy R. H. Regnault and William W. Hay, Jr.

In Vitro Models for Studying Trophoblast Transcellular Transport
Claudia J. Bode, Hong Jin, Erik Rytting, Peter S. Silverstein, Amber M. Young, and Kenneth L. Audus

In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Plasma Membrane Vesicles
Jocelyn D. Glazier and Colin P. Sibley

In Vitro Methods for Studying Human Placental Amino Acid Transport: Placental Villous Fragments
Susan L. Greenwood and Colin P. Sibley

Methods for Investigating Placental Fatty Acid Transport
Yan Xu, Thomas J. Cook, and Gregory T. Knipp

Heterologous Expression Systems for Studying Placental Transporters
Vadivel Ganapathy, You-Jun Fei, and Puttur D. Prasad

Analysis of Trophoblast Giant Cell Steroidogenesis in Primary Cultures
Noa Sher and Joseph Orly

Establishment of an ELISA for the Detection of Native Bovine Pregnancy-Associated Glycoproteins Secreted by Trophoblast Binucleate Cells
Jonathan A. Green and R. Michael Roberts

Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands
Heiner Muller and Michael J. Soares

Bacterial Expression of Prolactin Family Proteins
Arieh Gertler

Analysis of Placental Regulation of Hematopoiesis
Beiyan Zhou and Daniel I. H. Linzer

Methods for Studying Interferon Tau Stimulated Genes
Fuller W. Bazer and Thomas E. Spencer

Part V. Analysis of Placenta Adaptation to Disease

Reduced Uterine Perfusion Pressure (RUPP) Model for Studying Cardiovascular-Renal Dysfunction in Response to Placental Ischemia
Joey P. Granger, B. Babbette D. LaMarca, Kathy Cockrell, Mona Sedeek, Charles Balzi, Derrick Chandler, and William Bennett

In Vivo Rat Model of Preeclampsia
S. Ananth Karumanchi and Isaac E. Stillman

A Novel Mouse Model for Preeclampsia by Transferring Activated Th1 Cells into Normal Pregnant Mice
Ana Claudia
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Review Text

"...will provide an excellent laboratory resource for years to come." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journal

"...an important contribution to the literature, and I recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journal
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Review quote

"...will provide an excellent laboratory resource for years to come." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journal





"...an important contribution to the literature, and I recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy." -Weighted Numerical Score: 92 - 4 Stars!-Doody's Health Science Book Review Journal
show more