Phage Display of Peptides and Proteins : A Laboratory Manual
Both novices and experts should benefit from this step-by-step discussion of phage display protocols. It provides a review of the literature and outlines the strategies for maximizing the successful application of phage display technology to research. It contains the up-to-date protocols for preparing peptide affinity reagents, mono-clonal antibodies and evolved proteins.
- Spiral bound | 352 pages
- 200.66 x 241.3 x 20.32mm | 680.39g
- 23 Oct 1996
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Biology of the filamentous bacteriophage, B. Webster; principles and applications of phage display, B.K. Kay and R.H. Hoess; vectors for phage display, N.B. Adey, S.J. McConnell, and B.K. Kay; construction of random peptide libraries in bacteriophage M13, A.B. Sparks, N.B. Adey, J. Beasley, and B.K. Kay; construction and screening of antibody display libraries, J. McCafferty and K. Johnson; phagemid-displayed peptide libraries, D. Dottavio and J. Winter; phage libraries displaying random peptides derived from a target sequence, D.H. du Plessis and F. Jordaan; display and selection of proteins on genetic packages, R.C. Ladner; a cDNA cloning system based on filamentous phage - selection and enrichment of functional gene products by protein/ligand interactions made possible by linkage of recognition and replication functions, M. Suter, M. Foti, M. Ackermann, and R. Crameri; multicombinatorial libraries and combinatorial infection - practical considerations for vector design, R. Sudoyer; use of filamentous virus particles as immunogens, R. Perham; screening phage displayed random peptide libraries, A.B. Sparks, N.B. Adey, S. Cwirla, and B.K. Kay; substrate phage, D. Matthews; phage display - factors affecting planning efficiency, J. McCafferty; preparation of second generation phage libraries, N.B. Adey, W.P.C. Stemmer, and B.K. Kay; nonradioactive sequencing of random peptide recombinant phage, B.L. Masecar, C.A. Dadd, G.A. Baumbach, and D.J. Hammond; measurement of peptide binding affinities using fluorescence polarization, T. Burke, R. Bolger, W. Checovich, and R. Lowery.