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Phage Display in Biotechnology and Drug Discovery, Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition, the book discusses current theories, principles, and methods in the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug more

Product details

  • Paperback
  • 155.58 x 234.95mm
  • CRC Press
  • English
  • New edition
  • 1138894672
  • 9781138894679

About Sachdev S. Sidhu

Edited by Sachdev S. Sidhu, PhD, professor, Department of Molecular Genetics, University of Toronto, Ontario, Canada C. Ronald Geyer, PhD, professor, Department of Pathology, University of Saskatchewan, Canadashow more

Table of contents

Filamentous Bacteriophage Structure and BiologyDiane J. Rodi, Suneeta Mandava, and Lee MakowskiVectors and Modes of DisplayValery A. Petrenko and George P. SmithMethods for the Construction of Phage-Displayed LibrariesFrederic A. Fellouse and Gabor PalSelection and Screening StrategiesMark S. DennisLeveraging Synthetic Phage-Antibody Libraries for Panning on the Mammalian Cell SurfaceJelena Tomic, Megan McLaughlin, Traver Hart, Sachdev Sidhu, and Jason MoffatPhage Libraries for Developing Antibody-Targeted Diagnostics and VaccinesNienke E. van Houten and Jamie K. ScottExploring Protein-Protein Interactions Using Peptide Libraries Displayed on PhageKurt DeshayesSubstrate Phage DisplayShuichi OhkuboMapping Intracellular Protein NetworksMalgorzata E. Kokoszka, Zhaozhong Han, Ece Karatan, and Brian K. KayHigh-Throughput and High-Content Screening Using PeptidesRobert O. Carlson, Robin Hyde-Deruyscher, and Paul T. HamiltonEngineering Protein Folding and StabilityMihriban Tuna and Derek N. WoolfsonIdentification of Natural Protein-Protein Interactions with cDNA LibrariesReto Crameri, Claudio Rhyner, Michael Weichel, Sabine Fluckiger, and Zoltan KonthurMapping Protein Functional EpitopesSara K. Avrantinis and Gregory A. WeissSelections for Enzymatic CatalystsJulian Bertschinger, Christian Heinis, and Dario NeriAntibody Humanization and Affinity Maturation Using Phage DisplayJonathan S. Marvin and Henry B. LowmanAntibody Libraries from Immunized RepertoiresJody D. Berry and Mikhail PopkovNaive Antibody Libraries from Natural RepertoiresClaire L. Dobson, Ralph R. Minter, and Celia P. Hart-ShorrockSynthetic Antibody LibrariesFrederic A. Fellouse and Sachdev S. SidhuEngineering Antibody Fragments for Intracellular ApplicationsJianghai Liu and C. Ronald Geyershow more