PCR Protocols

PCR Protocols : A Guide to Methods and Applications

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The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.
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Product details

  • Hardback | 482 pages
  • 159 x 235 x 25.4mm | 910g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0123721806
  • 9780123721808

Review quote

"For an up-to-date, definitive discussion of technical variations and the wide range of known applications, PCR Protocols, A Guide to Methods and Applications is an invaluable reference. This work must, by its nature, be definitive; the first three editors are from Cetus, the corporation... The editors have done well to give this work a surprisingly cohesive feel... This manual is an excellent summary of PCR to date. The index is good and the reference citations alone would save an investigator new to the field countless hours in the library. The prose and 'cookbook' presentation of the procedures are clear at an appropriate level for anyone with a background in more conventional molecular techniques. While PCR is in its infancy, this work will certainly be updated many times and by many others, however as a reflection of the scope of PCR to date, it will not be replaced or rendered obsolete." --APPLIED CYTOGENETICS

"PCR Protocols is an excellent lab manual for making PCR work... Most of the articles are written with numbered, step-by-step procedures and have reagent information, down to the sequences of the oligonucleotides used. Even the comb binding of the book makes it suitable for bench-top use... The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work... The 30 or so applications articles give the experienced reader a good overview of the power and utility of PCR. In summary, if you have questions about PCR, PCR Protocols probably has the answers." --TIG

"This volume owes its value to the expertise of its contributors, all specialists of international renown in the developing field of PCR... This book is an excellent compilation for all who, having a general background in molecular biology, wish to practice PCR... No user of the PCR technique could wish to miss it." --TIBTECH

"The fifty-five chapters presented in this guide to the methods and applications of PCR provide detailed experimental protocols. They are written by researchers who use PCR successfully in their laboratory and who do not shy away from pointing out the potential pitfalls. This extremely useful publication of PCR Protocols goes far beyond the simple amplification of nucleic acids... A well-organized index is provided. This book will make it more difficult to find an excuse for not using the PCR technique." --AMERICAN JOURNAL OF PHYSIOLOGY
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Table of contents

[Contents in a condensed version]
Basic Methodology
Amplification of genomic DNA targets
Optimization of PCR reaction and design of primers
Nonradioactive detection of PCR products
mRNA quantitation by PCR
Cloning PCR products
Contamination and product carry-over
PCR from paraffin sections
Amplifying ancient DNA
Research Applications
Direct DNA sequencing of PCR products
DNA footprinting
Detection of rare mRNAs
Site-directed mutagenesis
Screening DNA libraries
Amplification of flanking DNA sequences
Genetics and Evoltion
Tissue typing and disease susceptibility
Haplotype analysis of single cells
Population biology
Multiplex DNA amplification
Diagnostics and Forensics
HIV detection
HTLV I and II detection
Screening for novel viruses
Detection of oncogenic mutations
Diagnosis of retinoblastoma
Individual identification
Instruments and Supplies
Organizing a laboratory for PCR work
Low-cost air-driven cycling oven
Basic equipment and supplies for the PCR lab
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